Overview
Product nameY14 protein
Protein descriptionRecombinant full length Human Y14 (amino acids 1-174) with a C terminal His tag; 182 amino acids with the tag, predicted mwt: 20.9 kDa (UniProt ID = Q9Y5S9).
Uniprot accessionQ9Y5S9
Molecular weight20.900kDa inclusive of tags
Protein length174 amino acids
Expression hostE. coli
Properties
Purity> 90
% by SDS-PAGE
Purification notesab105606 is purified using conventional chromatography techniques.
Mass spectrometryMALDI-TOF
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
Concentration information loading...
SequenceMADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK NIHLNLDRRT GYLKGYTLVE YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRRLEHHHH HH
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab105606
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
| MS |
MS: Use at an assay dependent dilution. |
Protein info
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Alternative names
Binder of OVCA1 1Binder of OVCA1-1BOV 1
BOV 1ABOV 1BBOV 1CBOV-1BOV1BOV1ABOV1BBOV1CHSPC 114HSPC114MDS 014MDS014RBM 8RBM 8ARBM 8BRBM8rbm8aRBM8A_HUMANRBM8BRibonucleoprotein RBM 8Ribonucleoprotein RBM 8ARibonucleoprotein RBM8Ribonucleoprotein RBM8ARNA binding motif protein 8RNA binding motif protein 8ARNA binding motif protein 8BRNA binding protein 8ARNA binding protein Y14RNA-binding motif protein 8ARNA-binding protein 8ARNA-binding protein Y14ZNRPZRNP 1ZRNP1
see all
FunctionComponent of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway.
Tissue specificityUbiquitous.
Sequence similaritiesBelongs to the RBM8A family.
Contains 1 RRM (RNA recognition motif) domain.
Cellular localizationNucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
References for Y14 protein (ab105606)
ab105606
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - Y14 protein (ab105606)15% SDS-PAGE showing ab105606 (3 µg) at approximately 20.9 kDa.