You have changed your country from to
. Please be aware that this will change the currency in the purchasing process.
- Primary antibodies
- Secondary antibodies
-
Proteins & Peptides
-
A-Z
- Full length proteins
- Partial proteins
- Peptides
-
Enzymes
- MMPs
- Caspases
- Kinases and transferases
- Signal transduction related
-
Cytokines and growth factors
- Cytokines
- Growth factors by family
-
Human proteins
- Human expressed
- Barley expressed
- Wheat expressed
- Fc fusion proteins
- Ig proteins
-
His-tagged Proteins by research area
- Cancer
- Cardiovascular
- Epigenetics & Nuclear Signaling
- Cell Biology
- Immunology
- Neuroscience
- Signal Transduction
- Stem Cells
-
Microbiology and virology
- All microbiology and viral proteins
- DNA virus proteins
- RNA viral proteins
- All Hepatitis virus proteins
- HIV proteins
- HSV, Measles and Influenza proteins
- Bacterial toxins and chaperones
-
Resources
- Advantages of expression in barley
-
-
ELISA Kits
-
A-Z
- ELISA Kits
- ELIPAIR Kits
- ELISPOT Kits
- FLUOROSPOT Kits
-
ELISA development
- Matched antibody pairs
-
Resources
- Immunoassay technical know-how
- ELISA and ELIPAIR
- ELISPOT and FLUOROSPOT
-
ELISA Kits by protein family
- Adaptive immune components
- Adhesion molecules
- Angiogenic factors
- Apoptosis markers
- Atherosclerotic proteins
- Blood cell antigens
- Cancer metabolism proteins
- Cardiovascular proteins
- CD markers
- Cell cycle proteins
- Complement activation
- Cytokines and cytokine receptors
- Developmental proteins
- Granzymes and perforins
- Growth factors/hormones
- Immunoglobulins
- Oncoproteins
- Pathogen specific immunoglobulins
- Protease inhibitors
- Signal transduction proteins
- Sub cellular markers
- Tumor biomarkers
-
-
Assay Kits
-
A-Z
- Apoptosis kits
- Epigenetic kits
- Metabolism kits
- ChIP kits
-
Metabolism kits by analyte
- Alcohol
- Amino acids
- Calcium
- Cell viability and senescence
- Coenzymes and cofactors
- Creatinine
- Inorganic ions
- Intermediary metabolism
- Lipid metabolism
- Nitric oxide
- Phosphates
- Sugars
- Oxidative stress
- Other metabolism assays
-
Apoptosis kits by analyte
- Annexin V
- Calpain
- Caspases
- Cathepsins
- DNA fragmentation
- Transmembrane potential
- Other Apoptosis assays
-
Epigenetic Kits by modification
- DNA modification and methylation
- Histone demethylation
- Histone methylation activity
- Sample Preparation
- Histone methylation quantification
- Histone acetylation and deacetylation
- Histone phosphorylation
- Sumoylation
-
-
Other Kits
-
A-Z
- All kits
-
Antibody labeling
- EasyLink antibody conjugation kits
-
Cell fractionation
- Cell fractionation kits A-Z
-
Cell Staining Kits
- CytoPainter kits
-
-
Biochemicals
A-Z
- By pharmacology
- By research area
- By chemical type
- By product range
Receptors & Transporters
- Adenosine
- Adrenoceptors
- Cannabinoid
- Catalytic Receptors
- Dopamine
- GABA
- Glutamate
- Glycine
- Histamine
- Imidazoline
- Melatonin
- Muscarinic
- Nicotinic
- Nuclear Receptors
- Opioids
- Peptide Receptors
- Purinergics
- Serotonin
- Transporters
-
Immunodetection
-
IHC Kits
- EXPOSE biotin-free detection kits
- Biotin-Streptavidin detection kits
- Endogenous avidin/biotin blocking systems
- Substrate kits
- Mouse on Mouse detection kit
- BrdU IHC detection kit
-
Secondary antibodies
- Advanced search
-
Reagents and buffers
- Buffers
- Chromogens and enhancers
- Diluents
- Epitope recovery and pretreatments
- Fluorescent ligands
- Mounting media
- PAP pen
- Serum
- Substrates
-
-
Controls
-
A-Z
- Isotype controls
- Loading controls
- Lysates
-
Isotype controls by host species
- Chicken
- Goat
- Hamster (Armenian and Syrian)
- Mouse
- Rabbit
- Rat
- Sheep
-
Lysates by type
- 293T transfected
- Cytoplasmic
- Membrane
- Nuclear
- Tissue
- Total protein
- Whole cell
-
Loading controls
- Loading controls by protein target
-
Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Anti-YAP1 antibody [EP1674Y] (ab52771)
Product partner
Related products
-
Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab52771? Please let us know so that we can cite the reference in this datasheet
ab52771 has been referenced in 3 publications.
- Fujii M et al. TGF-ß synergizes with defects in the Hippo pathway to stimulate human malignant mesothelioma growth. J Exp Med 209:479-94 (2012). WB; Human. PubMed: 22329991
- Murakami H et al. LATS2 is a tumor suppressor gene of malignant mesothelioma. Cancer Res 71:873-83 (2011). WB, ICC/IF, IHC-P; Human. PubMed: 21245096
- Kang W et al. Yes-associated protein 1 exhibits oncogenic property in gastric cancer and its nuclear accumulation associates with poor prognosis. Clin Cancer Res 17:2130-9 (2011). IHC. PubMed: 21346147
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - YAP1 antibody [EP1674Y] (ab52771)](/ps/datasheet/Images/52/ab52771/ab52771_1.jpg)
Western blot - YAP1 antibody [EP1674Y] (ab52771)
Anti-YAP1 antibody [EP1674Y] (ab52771) at 1/50000 dilution + Hela cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 65 kDa
Observed band size : 65 kDa
![Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y] (ab52771)](/ps/datasheet/Images/52/ab52771/ab52771_2.jpg)
Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y] (ab52771)
Immunohistochemical staining of YAP1 in paraffin embedded human breast carcinoma tissue using ab52771 at a 1/100 dilution.
![Western blot - YAP1 antibody [EP1674Y] (ab52771)](/ps/datasheet/images/52/ab52771/YAP1-Primary-antibodies-ab52771-4.jpg)
Western blot - YAP1 antibody [EP1674Y] (ab52771)
developed using the ECL technique
Predicted band size : 65 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
All lanes are ab52771 at a 1/1000 dilution plus whole cell lysate prepared from U87MG tumour cell line, treated with DMSO or 1uM &10uM LY294002, 50µg positive control loaded.Primary antibody was incubated for 16 hours at 4°C.Blocking step was performed using 5% milk for 1 hour at 23°C.Secondary used was a goat polyclonal conjugated to HRP, at a 1/10,000 dilution.
Image courtesy of an anonymous Abreview.
![Flow Cytometry - YAP1 antibody [EP1674Y] (ab52771)](/ps/datasheet/images/52/ab52771/YAP1-Primary-antibodies-ab52771-5.jpg)
Flow Cytometry - YAP1 antibody [EP1674Y] (ab52771)
Overlay histogram showing HeLa cells stained with ab52771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52771, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![YAP1 antibody [EP1674Y] for Western blot in Human (52771)](/ps/datasheet/images/52/ab52771/YAP1-Primary-antibodies-ab52771-3.jpg)
YAP1 antibody [EP1674Y] for Western blot in Human (52771)
1
Events
Talk to us
Special offers
© 1998-2012 Abcam plc. All rights reserved.
United States
0
- A - C
- Albania
- Algeria
- Antigua and Barbuda
- Argentina
- Armenia
- Aruba
- Australia
- Austria
- Azerbaijan
- Bahamas
- Bahrain
- Bangladesh
- Barbados
- Belarus
- Belgium
- Belize
- Bermuda
- Bolivia
- Bosnia and herzegovina
- Botswana
- Brazil
- Brunei darussalam
- Bulgaria
- Cambodia
- Cameroon
- Canada
- Cayman islands
- Chile
- China
- Colombia
- Costa rica
- Cote d'ivoire
- Croatia
- Cyprus
- Czech Republic
- D - I
- Denmark
- Dominica
- Dominican republic
- Ecuador
- Egypt
- El salvador
- Estonia
- Ethiopia
- Finland
- France
- French Guiana
- Gabon
- Gambia
- Georgia
- Germany
- Ghana
- Greece
- Grenada
- Guadeloupe
- Guam
- Guatemala
- Guyana
- Haiti
- Honduras
- Hong Kong
- Hungary
- Iceland
- India
- Indonesia
- Ireland
- Israel
- Italy
- J - O
- Jamaica
- Japan
- Jordan
- Kazakhstan
- Kenya
- Korea, Rep of
- Kuwait
- Kyrgystan
- Latvia
- Lebanon
- Lithuania
- Luxembourg
- Macau
- Macedonia
- Madagascar
- Malawi
- Malaysia
- Malta
- Martinique
- Mexico
- Monaco
- Morocco
- Nepal
- Netherlands
- Netherlands Ant
- New caledonia
- New Zealand
- Nicaragua
- Nigeria
- Norway
- Oman
Call 01223 696 000 or contact us