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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Anti-YAP1 antibody [EP1674Y] (ab52771)
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab52771 for help.
There are no answered questions for ab52771
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - YAP1 antibody [EP1674Y] (ab52771)](/ps/datasheet/Images/52/ab52771/ab52771_1.jpg)
Western blot - YAP1 antibody [EP1674Y] (ab52771)
Anti-YAP1 antibody [EP1674Y] (ab52771) at 1/50000 dilution + Hela cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 65 kDa
Observed band size : 65 kDa
![Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y] (ab52771)](/ps/datasheet/Images/52/ab52771/ab52771_2.jpg)
Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y] (ab52771)
Immunohistochemical staining of YAP1 in paraffin embedded human breast carcinoma tissue using ab52771 at a 1/100 dilution.
![Western blot - YAP1 antibody [EP1674Y] (ab52771)](/ps/datasheet/images/52/ab52771/YAP1-Primary-antibodies-ab52771-4.jpg)
Western blot - YAP1 antibody [EP1674Y] (ab52771)
developed using the ECL technique
Predicted band size : 65 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
All lanes are ab52771 at a 1/1000 dilution plus whole cell lysate prepared from U87MG tumour cell line, treated with DMSO or 1uM &10uM LY294002, 50µg positive control loaded.Primary antibody was incubated for 16 hours at 4°C.Blocking step was performed using 5% milk for 1 hour at 23°C.Secondary used was a goat polyclonal conjugated to HRP, at a 1/10,000 dilution.
Image courtesy of an anonymous Abreview.
![Flow Cytometry - YAP1 antibody [EP1674Y] (ab52771)](/ps/datasheet/images/52/ab52771/YAP1-Primary-antibodies-ab52771-5.jpg)
Flow Cytometry - YAP1 antibody [EP1674Y] (ab52771)
Overlay histogram showing HeLa cells stained with ab52771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52771, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![YAP1 antibody [EP1674Y] for Western blot in Human (52771)](/ps/datasheet/images/52/ab52771/YAP1-Primary-antibodies-ab52771-3.jpg)
YAP1 antibody [EP1674Y] for Western blot in Human (52771)
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