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Anti-YAP1 antibody [EP1674Y] (ab52771)

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Overview

Product name

Anti-YAP1 antibody [EP1674Y]
See all YAP1 products (14) ...

Description

Rabbit monoclonal [EP1674Y] to YAP1

Specificity

This antibody reacts with YAP1.

Tested applications

WB, IP, Flow Cyt, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues near the C terminus of human YAP1.

Positive control

WB: Hela cell lysate IHC-P: Human breast carcinoma tissue

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 50M Tris glycine, 0.15M Sodium chloride, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1674Y

Isotype

IgG

Research areas

Cancer >> Signal transduction >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family

  • Western blot - YAP1 antibody [EP1674Y] (ab52771)Western blot - YAP1 antibody [EP1674Y] (ab52771) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y] (ab52771)Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y] (ab52771) image (enlarge)

  • Western blot - YAP1 antibody [EP1674Y] (ab52771)Western blot - YAP1 antibody [EP1674Y] (ab52771) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

Tissue specificity

Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).

Sequence similarities

Belongs to the YORKIE family.
Contains 2 WW domains.

Post-translational
modifications

Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.

Cellular localization

Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.

Target information above from: UniProt accessionP46937 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 65 kDa Yes associated protein antibody
  • 65 kDa Yes-associated protein antibody
  • YAp 1 antibody
  • YAP 65 antibody
  • YAP antibody
  • YAP1 antibody
  • YAP1_HUMAN antibody
  • YAP2 antibody
  • YAP65 antibody
  • yes -associated protein delta antibody
  • Yes associated protein 1 65kDa antibody
  • Yes associated protein 1 antibody
  • Yes associated protein 2 antibody
  • yes associated protein beta antibody
  • YKI antibody
  • Yorkie homolog antibody
see all

Database links

Anti-YAP1 antibody [EP1674Y] images:

  Western blot - YAP1 antibody [EP1674Y] (ab52771)

Western blot - YAP1 antibody [EP1674Y] (ab52771)

Anti-YAP1 antibody [EP1674Y] (ab52771) at 1/50000 dilution + Hela cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size : 65 kDa
Observed band size : 65 kDa

  Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y] (ab52771)

Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y] (ab52771)

Immunohistochemical staining of YAP1 in paraffin embedded human breast carcinoma tissue using ab52771 at a 1/100 dilution.

  Western blot - YAP1 antibody [EP1674Y] (ab52771)

Western blot - YAP1 antibody [EP1674Y] (ab52771)


developed using the ECL technique

Predicted band size : 65 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)


Exposure time : 1 minute

All lanes are ab52771 at a 1/1000 dilution plus whole cell lysate prepared from U87MG tumour cell line, treated with DMSO or 1uM &10uM LY294002, 50µg positive control loaded.
Primary antibody was incubated for 16 hours at 4°C.
Blocking step was performed using 5% milk for 1 hour at 23°C.
Secondary used was a goat polyclonal conjugated to HRP, at a 1/10,000 dilution.

Image courtesy of an anonymous Abreview.

See Abreview

  Flow Cytometry - YAP1 antibody [EP1674Y] (ab52771)

Flow Cytometry - YAP1 antibody [EP1674Y] (ab52771)

Overlay histogram showing HeLa cells stained with ab52771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52771, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-YAP1 antibody [EP1674Y] (ab52771)

This product has been referenced in:

  • Murakami Het al. LATS2 is a tumor suppressor gene of malignant mesothelioma. Cancer Res 71:873-83 (2011). WB, ICC/IF, IHC-P; Human.Read more (PubMed: 21245096) »
  • Kang Wet al. Yes-associated protein 1 exhibits oncogenic property in gastric cancer and its nuclear accumulation associates with poor prognosis. Clin Cancer Res 17:2130-9 (2011). IHC.Read more (PubMed: 21346147) »

See all 2 publications for this product

Publishing research using ab52771? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"