YAP1 antibody [EP1674Y] (ab52771)

Product Name 

YAP1 antibody [EP1674Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1674Y] to YAP1

Immunogen 

A synthetic peptide corresponding to residues near the C terminus of human YAP1.

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Specificity 

This antibody reacts with YAP1.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/25.
ICC/IF: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
IP: 1/70.
WB: 1/25000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 65 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Hela cell lysate
IHC-P: Human breast carcinoma tissue

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cancer >> Signal transduction >> Other
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family

Relevance 

YAP1 (also known as Yes associated protein 1) was originally identified as a transcription factor that binds to the SH3 domain of the YES kinase (a Src protein kinase). More recently it has been identified as a candidate oncogene that promotes tumorigenesis in many different types of cancer.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1674Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 50M Tris glycine, 0.15M Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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