Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
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ab76252 at 1/100 dilution staining YAP1 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/50000 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell lysates, treated with FBS + calyculin
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 65 - 75 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
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