Overview

  • Product nameAnti-YAP1 (phospho S127) antibody [EP1675Y]See all YAP1 primary antibodies ...
  • Description
    Rabbit monoclonal [EP1675Y] to YAP1 (phospho S127)
  • Specificityab76252 detects YAP1 phosphorylated at Serine 127.
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Ser127 of human YAP1

  • Positive control
    • HeLa cell lysates, untreated or treated with FBS + calyculin; human breast carcinoma tissue.
  • General notesProduced under U.S. Patent No. 5,675,063

Properties

Applications

Our Abpromise guarantee covers the use of ab76252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 - 75 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionTranscriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).
    • Tissue specificityIncreased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
    • Sequence similaritiesBelongs to the YORKIE family.
      Contains 2 WW domains.
    • Post-translational
      modifications
      Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.
    • Cellular localizationCytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.
    • Information by UniProt
    • Database links
    • Alternative names
      • 65 kDa Yes associated protein antibody
      • 65 kDa Yes-associated protein antibody
      • YAp 1 antibody
      • YAP 65 antibody
      • YAP antibody
      • YAP1 antibody
      • YAP1_HUMAN antibody
      • YAP2 antibody
      • YAP65 antibody
      • yes -associated protein delta antibody
      • Yes associated protein 1 65kDa antibody
      • Yes associated protein 1 antibody
      • Yes associated protein 2 antibody
      • yes associated protein beta antibody
      • YKI antibody
      • Yorkie homolog antibody
      see all

    Anti-YAP1 (phospho S127) antibody [EP1675Y] images

    • ab76252 at 1/100 dilution staining YAP1 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/50000 dilution

      Lane 1 : HeLa cell lysates, untreated
      Lane 2 : HeLa cell lysates, treated with FBS + calyculin

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 65 - 75 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/5000 dilution

      Lane 1 : C6 treated with Calyculin A
      Lane 2 : NIH/3T3 treated with Calyculin A

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size : 65 - 75 kDa
      Observed band size : 65-75 kDa (why is the actual band size different from the predicted?)

    References for Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252)

    This product has been referenced in:
    • Fujii M  et al. TGF-ß synergizes with defects in the Hippo pathway to stimulate human malignant mesothelioma growth. J Exp Med 209:479-94 (2012). WB ; Human . Read more (PubMed: 22329991) »
    • Beverdam A  et al. Yap Controls Stem/Progenitor Cell Proliferation in the Mouse Postnatal Epidermis. J Invest Dermatol : (2012). Read more (PubMed: 23190885) »

    See all 3 Publications for this product

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