YAP1 (phospho S127) antibody [EP1675Y] (ab76252)

Product Name 

YAP1 (phospho S127) antibody [EP1675Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1675Y] to YAP1 (phospho S127)

Immunogen 

A phospho specific peptide corresponding to residues surrounding Ser127 of human YAP1

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Specificity 

ab76252 detects YAP1 phosphorylated at Serine 127.

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 - 75 kDa).

Is unsuitable for Flow Cyt, ICC or IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysates, untreated or treated with FBS + calyculin; human breast carcinoma tissue.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cancer >> Signal transduction >> Other
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family

Relevance 

YAP1 (also known as Yes associated protein 1) was originally identified as a transcription factor that binds to the SH3 domain of the YES kinase (a Src protein kinase). More recently it has been identified as a candidate oncogene that promotes tumorigenesis in many different types of cancer.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1675Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

Notes 

Produced under U.S. Patent No. 5,675,063

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