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All lanes : Anti-YAP1 (phospho Y357) antibody (ab62751) at 1/2000 dilution
Lane 1 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl
Lane 2 : Whole cell lysates of untransfected HEK-293T cells
Lane 3 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl with YAP1 peptide (human 351-362) at 20 µg/ml
Lane 4 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl with phospho-YAP1 (human 351-362 [phospho Y357]) immunizing peptide at 20 µg/ml
Secondary
Goat Anti-Rabbit IgG, Peroxidase conjugate
Predicted band size : 65 kDa
Observed band size : 65 kDa
Additional bands at : 100 kDa,70 kDa. We are unsure as to the identity of these extra bands.
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