Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-YARS2 antibody - Aminoterminal end
See all YARS2 products (2) ...
Description
Rabbit polyclonal to YARS2 - Aminoterminal end
Tested applications
IHC-P, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the N-terminal region of human YARS2, conjugated to KLH.
Positive control
K562 cell line lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/10 - 1/50.
WB: 1/50 - 1/100.Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Relevance
YARS2 is a tRNA synthetase and catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Cellular localization
Mitochondrial matrix
Alternative names
- CGI 04 antibody
- FLJ13995 antibody
- mt TyrRS antibody
see all
Database links
- Entrez Gene: 51067 Human
- Omim: 610957 Human
- SwissProt: Q9Y2Z4 Human
Anti-YARS2 antibody - Aminoterminal end images:
Western blot - YARS2 antibody - Aminoterminal end (ab71419)
Anti-YARS2 antibody - Aminoterminal end (ab71419) at 1/60 dilution + K562 cell line lysate at 35 µg
Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-YARS2 antibody - Aminoterminal end(ab71419)
ab71419 at 1/50 dilution staining YARS2 - Aminoterminal end in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-YARS2 antibody - Aminoterminal end (ab71419)
ab71419 has not yet been referenced specifically in any publications.
Publishing research using ab71419? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"