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Western blot - YARS2 antibody - Aminoterminal end (ab71419)
Western blot - YARS2 antibody - Aminoterminal end (ab71419)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-YARS2 antibody - Aminoterminal end(ab71419)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-YARS2 antibody - Aminoterminal end(ab71419)
(enlarge)


Product Name 

YARS2 antibody - Aminoterminal end

 

See all YARS2 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to YARS2 - Aminoterminal end

Immunogen 

Synthetic peptide from the N-terminal region of human YARS2, conjugated to KLH.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/10 - 1/50.
WB: 1/50 - 1/100. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

K562 cell line lysate.

Cellular localization 

Mitochondrial matrix

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria

Relevance 

YARS2 is a tRNA synthetase and catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).

 

Alternative names YARS2 antibody - Aminoterminal end (ab71419)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

51067    (Human)

Omim

    

610957    (Human)

SwissProt

    

Q9Y2Z4    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Ammonium Sulphate Precipitation

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this YARS2 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance!


See below for YARS2 antibody - Aminoterminal end images, references, products related to ab71419 and other tools.

 

YARS2 antibody - Aminoterminal end images:

Product image  Western blot - YARS2 antibody - Aminoterminal end (ab71419)

Western blot - YARS2 antibody - Aminoterminal end (ab71419)

YARS2 antibody - Aminoterminal end (ab71419) at 1/60 dilution + K562 cell line lysate at 35 µg

Predicted band size : 53 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-YARS2 antibody - Aminoterminal end(ab71419)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-YARS2 antibody - Aminoterminal end(ab71419)

ab71419 at 1/50 dilution staining YARS2 - Aminoterminal end in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

 
 

Search PubMed (MEDLINE) for references to YARS2

 

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Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

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