|
|
|
|
Western blot - YARS2 antibody (ab68725)

(enlarge)
|
|
|
|
|
Product Name
|
YARS2 antibody
|
| |
See all YARS2 antibodies (2)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse polyclonal to YARS2
|
|
Immunogen
|
Full length Human protein, (NP_001035526.1, 477a.a.)
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions WB: 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 53 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
YARS2 transfected 293T cell lysate
|
|
Cellular localization
|
Mitochondrial matrix
|
|
Research areas
|
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
|
|
Relevance
|
YARS2 is a tRNA synthetase and catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window
|
|
Raised in
|
Mouse
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Whole antiserum
|
|
Storage buffer
|
Preservative: None Constituents: Whole serum
|
|
Form
|
Liquid
|
|
Concentration
|
Concentration not determined - why is this?
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this YARS2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for YARS2 antibody images, references, products related to ab68725 and other tools.
|
| |
YARS2 antibody images:
|
Western blot - YARS2 antibody (ab68725)
All lanes : YARS2 antibody (ab68725) at 1/500 dilution
Lane 1 : YARS2-transfected 293T cells lysate Lane 2 : Non transfected 293T cells lysate
Lysates/proteins at 25 µg per lane.
Secondary Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size : 53 kDa Observed band size : 45 kDa (why is the actual band size different from the predicted?)
|
| |
| |
|
Search PubMed (MEDLINE) for references to YARS2
|
| |
YARS2 antibody - more information
|
Customer reviews (feedback) regarding YARS2 antibody |
Customer FAQs regarding YARS2 antibody |
Protocols for YARS2 antibody |
Price and availability of products related to YARS2 antibody |
| |
YARS2 antibody - related products:
|
|
|
| |
YARS2 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about YARS2 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"