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Western blot - YARS2 antibody (ab68725)
Western blot - YARS2 antibody (ab68725)
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Product Name 

YARS2 antibody

 

See all YARS2 antibodies (2)...

Product type 

Primary antibodies

Description 

Mouse polyclonal to YARS2

Immunogen 

Full length Human protein, (NP_001035526.1, 477a.a.)

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 53 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

YARS2 transfected 293T cell lysate

Cellular localization 

Mitochondrial matrix

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria

Relevance 

YARS2 is a tRNA synthetase and catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).

 

Alternative names YARS2 antibody (ab68725)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

51067    (Human)

Omim

    

610957    (Human)

SwissProt

    

Q9Y2Z4    (Human)

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole serum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this YARS2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for YARS2 antibody images, references, products related to ab68725 and other tools.

 

YARS2 antibody images:

Product image  Western blot - YARS2 antibody (ab68725)

Western blot - YARS2 antibody (ab68725)

All lanes : YARS2 antibody (ab68725) at 1/500 dilution

Lane 1 : YARS2-transfected 293T cells lysate
Lane 2 : Non transfected 293T cells lysate

Lysates/proteins at 25 µg per lane.

Secondary
Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size : 53 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to YARS2

 

YARS2 antibody - more information

Customer reviews (feedback) regarding YARS2 antibody

Customer FAQs regarding YARS2 antibody

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Price and availability of products related to YARS2 antibody

 

YARS2 antibody - related products:

Products for Western blot

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Lysates

 

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