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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-YARS2 antibody
See all YARS2 products (2) ...
Description
Mouse polyclonal to YARS2
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length Human protein, (NP_001035526.1, 477a.a.)
Positive control
YARS2 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Western blot - YARS2 antibody (ab68725)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 45 kDa (predicted molecular weight: 53 kDa).
Target
Relevance
YARS2 is a tRNA synthetase and catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Cellular localization
Mitochondrial matrix
Alternative names
- CGI 04 antibody
- FLJ13995 antibody
- mt TyrRS antibody
see all
Database links
- Entrez Gene: 51067 Human
- Omim: 610957 Human
- SwissProt: Q9Y2Z4 Human
Anti-YARS2 antibody images:
Western blot - YARS2 antibody (ab68725)
All lanes : Anti-YARS2 antibody (ab68725) at 1/500 dilution
Lane 1 : YARS2-transfected 293T cells lysate
Lane 2 : Non transfected 293T cells lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size : 53 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
References for Anti-YARS2 antibody (ab68725)
ab68725 has not yet been referenced specifically in any publications.
Publishing research using ab68725? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"