Anti-YTHDC1 antibody (ab122340)
Key features and details
- Rabbit polyclonal to YTHDC1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-YTHDC1 antibody
See all YTHDC1 primary antibodies -
Description
Rabbit polyclonal to YTHDC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to internal sequence amino acids 69-160 (LVSKPLSSSV SNNKRIVSTK GKSATEYKNE EYQRSERNKR LDADRKIRLS SSASREPYKN QPEKTCVRKR DPERRAKSPT PDGSERIGLE VD) of Human YTHDC1
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Positive control
- IHC-P: Human esophagus, fallopian tube, duodenum, endometrium and testis tissues. WB: RT-4 and U-251 MG cell lysates. ICC/IF: HeLa and U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P | (5) |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
1/250 - 1/500. Predicted molecular weight: 85 kDa.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. Predicted molecular weight: 85 kDa. |
Target
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Function
May be part of a signal transduction pathway that influences splice site selection. -
Sequence similarities
Contains 1 YTH domain. -
Cellular localization
Nucleus. Localizes to a novel subnuclear structure, the YT bodies. - Information by UniProt
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Database links
- Entrez Gene: 91746 Human
- SwissProt: Q96MU7 Human
- Unigene: 175955 Human
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Alternative names
- KIAA1966 antibody
- Putative splicing factor YT521 antibody
- splicing factor YT521-B antibody
see all
Images
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All lanes : Anti-YTHDC1 antibody (ab122340) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Predicted band size: 85 kDa -
Paraffin-embedded human testis tissue stained for YTHDC1 using ab122340 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium tissue stained for YTHDC1 using ab122340 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human duodenum tissue stained for YTHDC1 using ab122340 at 1/500 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for YTHDC1 (green) using ab122340 at 4 µg/ml in ICC/IF.
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab122340 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122340, at a 1/400 dilution, staining YTHDC1 in paraffin-embedded Human esophagus tissue by immunohistochemistry.
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Immunohistochemical staining of Human fallopian tube shows strong nuclear positivity in glandular cells using ab122340
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab122340 has been referenced in 16 publications.
- Tian Y et al. Crosstalk between 5-methylcytosine and N6-methyladenosine machinery defines disease progression, therapeutic response and pharmacogenomic landscape in hepatocellular carcinoma. Mol Cancer 22:5 (2023). PubMed: 36627693
- Liang D et al. m6A reader YTHDC1 modulates autophagy by targeting SQSTM1 in diabetic skin. Autophagy 18:1318-1337 (2022). PubMed: 34657574
- Chen L et al. Nuclear m6 A reader YTHDC1 suppresses proximal alternative polyadenylation sites by interfering with the 3' processing machinery. EMBO Rep 23:e54686 (2022). PubMed: 36094741
- Porman AM et al. A single N6-methyladenosine site regulates lncRNA HOTAIR function in breast cancer cells. PLoS Biol 20:e3001885 (2022). PubMed: 36441764
- You A et al. TTC22 promotes m6A-mediated WTAP expression and colon cancer metastasis in an RPL4 binding-dependent pattern. Oncogene N/A:N/A (2022). WB ; Human . PubMed: 35798874