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Anti-YTHDF3 antibody (ab83716)

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Overview

Product name

Anti-YTHDF3 antibody
See all YTHDF3 products (2) ...

Description

Rabbit polyclonal to YTHDF3

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Cow, Dog, Zebrafish

Immunogen

Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (GEAAWSTAGD QPMPYLTTYG QMSNGEHHYI PDGVFSQPGA LGNTPPFLGQ) of Human YTHDF3 (NP_689971).

GEAAWSTAGD QPMPYLTTYG QMSNGEHHYI PDGVFSQPGA LGNTPPFLGQ

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Hela cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Other Antibodies >> Other Antibodies

  • Western blot - YTHDF3 antibody (ab83716)Western blot - YTHDF3 antibody (ab83716) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab83716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/312500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

YTHDF3 contains 1 YTH domain. The functions of YTHDF3 remain unknown.

Alternative names

  • FLJ31657 antibody
  • YTH domain family 3 antibody
  • YTH domain family member 3 antibody
  • YTH domain family protein 3 antibody
  • YTHDF 3 antibody
see all

Database links

Anti-YTHDF3 antibody images:

  Western blot - YTHDF3 antibody (ab83716)

Western blot - YTHDF3 antibody (ab83716)

Anti-YTHDF3 antibody (ab83716) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 64 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

Gel concentration: 12%

References for Anti-YTHDF3 antibody (ab83716)

ab83716 has not yet been referenced specifically in any publications.

Publishing research using ab83716? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"