Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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ab12132 has been referenced in 6 publications.
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Predicted band size : 49.7 kDa
Detection of YY1 by Western blot analysis. YY1 can be detected in nuclear extracts derived from Raji cells using ab12132 (lane 1) at a 1:1000 dilution. The signal corresponding to nuclear YY1 is eliminated by adding the immunizing peptide to the reaction mixture (lane 2).
ICC/IF image of ab12132 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12132, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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