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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-YY1 antibody - ChIP Grade
See all YY1 products (9) ...
Description
Rabbit polyclonal to YY1 - ChIP Grade
Tested applications
ChIP, ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
KLH-conjugated synthetic peptide selected from the region of aa 250-300 of human YY1 protein.
Positive control
human hepatocarcinoma tissue. HL60 cell lysate. mouse kidney tissue lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution. (PubMed: 19721712)
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500.Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Target
Function
Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
Sequence similarities
Belongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers.
Cellular localization
Nucleus matrix. Associated with the nuclear matrix.
Target information above from: UniProt accessionP25490
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- YY1 transcription factor antibody
- CF1 antibody
- Delta antibody
see all
Database links
- Entrez Gene: 7528 Human
- Entrez Gene: 22632 Mouse
- Omim: 600013 Human
- SwissProt: P25490 Human
- SwissProt: Q00899 Mouse
- Unigene: 388927 Human
- Unigene: 3868 Mouse
- Unigene: 458511 Mouse
Anti-YY1 antibody - ChIP Grade images:
Western blot - YY1 antibody (ab38422)
All lanes : Anti-YY1 antibody - ChIP Grade (ab38422) at 1/100 dilution
Lane 1 : HL60 cell lysate
Lane 2 : Mouse kidney tissue lysate
Predicted band size : 45 kDa
Observed band size : 45 kDa
Additional bands at : 30 kDa,65 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YY1 antibody (ab38422)
Immunohistochemical analysis of YY1 expression in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue using 1/50 ab38422.
References for Anti-YY1 antibody - ChIP Grade (ab38422)
This product has been referenced in:
- Song G & Wang L Nuclear receptor SHP activates miR-206 expression via a cascade dual inhibitory mechanism. PLoS One 4:e6880 (2009). ChIP.Read more (PubMed: 19721712) »
See 1 publication for this product
Publishing research using ab38422? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"