Recombinant Anti-YY1 antibody [EPR4651] (ab109228)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4651] to YY1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-YY1 antibody [EPR4651]
See all YY1 primary antibodies -
Description
Rabbit monoclonal [EPR4651] to YY1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human colon tissue; HeLa, Y79 and HuT-78 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4651 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
Target
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Function
Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. -
Sequence similarities
Belongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers. -
Cellular localization
Nucleus matrix. Associated with the nuclear matrix. - Information by UniProt
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Database links
- Entrez Gene: 7528 Human
- Entrez Gene: 22632 Mouse
- Entrez Gene: 24919 Rat
- Omim: 600013 Human
- SwissProt: P25490 Human
- SwissProt: Q00899 Mouse
- Unigene: 388927 Human
- Unigene: 3868 Mouse
see all -
Alternative names
- CF1 antibody
- Delta antibody
- Delta transcription factor antibody
see all
Images
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All lanes : Anti-YY1 antibody [EPR4651] (ab109228) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Y79 cell lysates
Lane 3 : HuT-78 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
Immunohistochemical analysis of YY1 in paraffin embedded Human colon tissue, using a 1/250 dilution of ab109228.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab109228 has been referenced in 24 publications.
- Chen X et al. TTN-AS1 accelerates the growth and migration of nasopharyngeal carcinoma cells via targeting miR-876-5p/NETO2. Mol Ther Oncolytics 24:535-546 (2022). PubMed: 35229031
- Sato N et al. Yin Yang 1 regulates ITGAV and ITGB1, contributing to improved prognosis of colorectal cancer. Oncol Rep 47:N/A (2022). PubMed: 35266011
- Mondal S et al. PROBER identifies proteins associated with programmable sequence-specific DNA in living cells. Nat Methods 19:959-968 (2022). PubMed: 35927480
- Zhu Y et al. The lncRNA NEAT1 Inhibits miRNA-216b and Promotes Colorectal Cancer Progression by Indirectly Activating YY1. J Oncol 2022:8130132 (2022). PubMed: 36262350
- Cao SJ et al. Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma. Oncol Lett 24:457 (2022). PubMed: 36380881