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Western blot - YY1 antibody (ab12132)
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Immunocytochemistry/ Immunofluorescence-YY1 antibody(ab12132)
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Product Name
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YY1 antibody
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See all YY1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to YY1
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Immunogen
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Synthetic peptide, corresponding to amino acids 109-123 of Human YY1.
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Reacts with
(species key)
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Hu, Ms, Rat, Mk
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Specificity
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ab12132 reacts with human YY1.
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 49.7 kDa). For optimal results, primary antibody incubations should be performed at 4°C.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Raji nuclear extract.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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YY1 is a ubiquitously distributed transcription factor belonging to the GLI Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide, 0.01% thimerosal
Constituents: 0.2% Gelatin, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Affinity purified.
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Form
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Liquid
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Concentration
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0.300 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this YY1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for YY1 antibody images, references, products related to ab12132 and other tools.
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YY1 antibody images:
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 Western blot - YY1 antibody (ab12132)
Predicted band size : 49.7 kDa
Detection of YY1 by Western blot analysis. YY1 can be detected in nuclear extracts derived from Raji cells using ab12132 (lane 1) at a 1:1000 dilution. The signal corresponding to nuclear YY1 is eliminated by adding the immunizing peptide to the reaction mixture (lane 2).
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Immunocytochemistry/ Immunofluorescence-YY1 antibody(ab12132)
ICC/IF image of ab12132 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12132, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for YY1 antibody (ab12132)
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This product has been used in: (two most recent references)
Souki SK et al. Arginine methylation of the ICP27 RGG box regulates ICP27 export and is required for efficient herpes simplex virus 1 replication. J Virol 83:5309-20 (2009). WB; Human. PubMed: 19321610
Kim SY et al. Juxtaposed Polycomb complexes co-regulate vertebral identity. Development 133:4957-68 (2006). PubMed: 17107999
If you publish research using ab12132 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to YY1
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YY1 antibody - more information
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Customer reviews (feedback) regarding YY1 antibody |
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Price and availability of products related to YY1 antibody |
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YY1 antibody - related products:
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YY1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about YY1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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