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Anti-YY1 antibody (ab43058)

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Overview

Product name

Anti-YY1 antibody
See all YY1 products (9) ...

Description

Rabbit polyclonal to YY1

Tested applications

ICC/IF, WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Sheep, Chicken, Cow

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human YY1.

(Peptide available as ab45370.)

Positive control

This antibody gave a positive signal in Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - YY1 antibody (ab43058)Western blot - YY1 antibody (ab43058) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - YY1 antibody (ab43058)Immunocytochemistry/ Immunofluorescence - YY1 antibody (ab43058) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YY1 antibody (ab43058)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YY1 antibody (ab43058) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab43058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 mg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 45 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.

Sequence similarities

Belongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers.

Cellular localization

Nucleus matrix. Associated with the nuclear matrix.

Target information above from: UniProt accessionP25490 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • YY1 transcription factor antibody
  • CF1 antibody
  • Delta antibody
  • Delta transcription factor antibody
  • INO80 complex subunit S antibody
  • INO80S antibody
  • NF E1 antibody
  • NF-E1 antibody
  • NFE1 antibody
  • OTTHUMP00000197459 antibody
  • Transcriptional repressor protein YY1 antibody
  • TYY1_HUMAN antibody
  • UCR motif DNA binding protein antibody
  • UCRBP antibody
  • Yin and yang 1 antibody
  • Yin and Yang 1 protein antibody
  • Yin Yang 1 antibody
  • Ying Yang 1 antibody
  • YY 1 antibody
  • YY 1 transcription factor antibody
  • YY-1 antibody
  • YY1 antibody
see all

Anti-YY1 antibody images:

  Western blot - YY1 antibody (ab43058)

Western blot - YY1 antibody (ab43058)

Anti-YY1 antibody (ab43058) at 2 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844) at 20 µg

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 45 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

The band observed at 58 kDa is consistent with the banding pattern seen in other commercially available antibodies to YY1.

  Immunocytochemistry/ Immunofluorescence - YY1 antibody (ab43058)

Immunocytochemistry/ Immunofluorescence - YY1 antibody (ab43058)

ICC/IF image of ab43058 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab43058, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HepG2 and MCF7 cells.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YY1 antibody (ab43058)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YY1 antibody (ab43058)

IHC image of YY1 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab43058, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-YY1 antibody (ab43058)

ab43058 has not yet been referenced specifically in any publications.

Publishing research using ab43058? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"