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Western blot - YY1 antibody (ab43058)
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Immunocytochemistry/ Immunofluorescence - YY1 antibody (ab43058)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YY1 antibody (ab43058)
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Product Name
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YY1 antibody
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See all YY1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to YY1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human YY1. (Peptide available as ab45370.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Chk, Cow, Shp
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 mg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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YY1 is a ubiquitously distributed transcription factor belonging to the GLI Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.600 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this YY1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for YY1 antibody images, references, products related to ab43058 and other tools.
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YY1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - YY1 antibody (ab43058)
ICC/IF image of ab43058 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab43058, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HepG2 and MCF7 cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YY1 antibody (ab43058)
IHC image of YY1 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab43058, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Search PubMed (MEDLINE) for references to YY1
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YY1 antibody - more information
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Customer reviews (feedback) regarding YY1 antibody |
Customer FAQs regarding YY1 antibody |
Protocols for YY1 antibody |
Price and availability of products related to YY1 antibody |
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YY1 antibody - related products:
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YY1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about YY1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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