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Predicted band size : 28.9 kDa
ab4468 works well on NuA4 purified fractions from yeast whole cell extracts. The NuA4 HAT complex was purified by tandem affinity (IgG and calmodulin) using a Epl1-TAP strain (another NuA4 subunit). 250ml yeast culture was used for the purification and 5ul of calmodulin elution (1/10 of the prep) was used in western with 1:1000 dilution of ab4468.
Jacques Cote
2
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