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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Yaf9 antibody
Description
Rabbit polyclonal to Yaf9
Tested applications
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Recombinant full length protein of S. cerevisiae Yaf9.
.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> INO80
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Western blot - Yaf9 antibody (ab4468)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 31 kDa (predicted molecular weight: 28.9 kDa).
Target
Relevance
Yeast homolog of the human leukemogenic protein AF9; member of a yeast chromatin modifying complex.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- Af9 antibody
- Protein AF9 homolog antibody
- Yaf 9p antibody
- Ynk7 antibody
Database links
- Entrez Gene: 855616 Saccharomyces cerevisiae
- SwissProt: P53930 Saccharomyces cerevisiae
Anti-Yaf9 antibody images:
Western blot - Yaf9 antibody (ab4468)
Predicted band size : 28.9 kDa
ab4468 works well on NuA4 purified fractions from yeast whole cell extracts. The NuA4 HAT complex was purified by tandem affinity (IgG and calmodulin) using a Epl1-TAP strain (another NuA4 subunit). 250ml yeast culture was used for the purification and 5ul of calmodulin elution (1/10 of the prep) was used in western with 1:1000 dilution of ab4468.
Jacques Cote
References for Anti-Yaf9 antibody (ab4468)
This product has been referenced in:
- Knutson BA & Hahn S Domains of Tra1 important for activator recruitment and transcription coactivator functions of SAGA and NuA4 complexes. Mol Cell Biol 31:818-31 (2011). WB; Saccharomyces cerevisiae.Read more (PubMed: 21149579) »
- Auger Aet al. Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants. Mol Cell Biol 28:2257-70 (2008). WB; Saccharomyces cerevisiae.Read more (PubMed: 18212047) »
See all 2 publications for this product
Publishing research using ab4468? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"