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Western blot - Yes1 antibody (ab61206)
Western blot - Yes1 antibody (ab61206)
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Product Name 

Yes1 antibody

 

See all Yes1 antibodies (3)...

Product type 

Primary antibodies

Description 

Mouse monoclonal to Yes1

Immunogen 

Recombinant full length protein with tag, corresponding to amino acids 1-544 of Human Yes1

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ELISA, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A431 whole cell lysate.

Cellular localization 

Cytoplasm; cytosol.
Note: In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies.

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family

Relevance 

The gene that codifies for Yes1 is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the SRC family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a pseudogene of it has been found on chromosome 22.

 

Alternative names Yes1 antibody (ab61206)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

7525    (Human)
22612    (Mouse)
24884    (Rat)

GeneCard

    

GC18M000711    (Human)

Omim

    

164880    (Human)

SwissProt

    

P07947    (Human)
Q04736    (Mouse)
Q3TJI7    (Mouse)
Q8C762    (Mouse)
Q8CBP1    (Mouse)
Q6AXQ3    (Rat)

Unigene

    

194148    (Human)
92532    (Rat)

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: PBS, pH 7.2

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Yes1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Yes1 antibody images, references, products related to ab61206 and other tools.

 

Yes1 antibody images:

Product image  Western blot - Yes1 antibody (ab61206)

Western blot - Yes1 antibody (ab61206)

Yes1 antibody (ab61206) at 1 µg/ml + A431 whole cell lysate at 50 µg/ml

Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size : 61 kDa
Observed band size : 61 kDa

 
 

Search PubMed (MEDLINE) for references to Yes1

 

Yes1 antibody - more information

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Price and availability of products related to Yes1 antibody

 

Yes1 antibody - related products:

Products for Western blot

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Lysates

 

Positive Controls for Yes1 antibody

ab7909

  

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

 

Compatible Secondaries for Yes1 antibody

ab2891

  

HRP polymer antibody

ab6563

  

Cy5 ® antibody

ab6726

  

Texas Red ® antibody

ab6728

  

HRP antibody

ab6729

  

Alkaline Phosphatase antibody

ab6785

  

FITC antibody

ab6788

  

Biotin antibody

ab7002

  

Phycoerythrin antibody

ab7018

  

Agarose antibody

ab27229

  

15nm Gold antibody

ab27230

  

10nm Gold antibody

ab27231

  

15nm Gold antibody

ab27233

  

5nm Gold antibody

ab27241

  

10nm Gold antibody

ab27242

  

20nm Gold antibody

ab27243

  

40nm Gold antibody

ab27244

  

5nm Gold antibody

ab60313

  

Chromeo™ 488 antibody

ab60316

  

Chromeo™ 546 antibody

ab60318

  

Chromeo™ 642 antibody

 

Other Yes1 Antibodies

See all Yes1 antibodies (3)...

 

Yes1 antibody - other tools:

Contact Abcam with a Technical Enquiry about Yes1 antibody

 

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