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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Overview
Product name
Anti-Yes1 (phospho Y537) antibody
See all Yes1 products (5) ...
Description
Rabbit polyclonal to Yes1 (phospho Y537)
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y537 of human YES1.
Positive control
Human hepatocarcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
First purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2 step procedure with the control and phosphorylated peptides. The phospho specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab60028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500.Predicted molecular weight: 61 kDa.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Post-translational
modifications
Autophosphorylated.
Cellular localization
Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies.
Target information above from: UniProt accessionP07947
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- c Yes antibody
- Cellular Yes 1 protein antibody
- Cellular Yes1 protein antibody
see all
Database links
- Entrez Gene: 7525 Human
- Omim: 164880 Human
- SwissProt: P07947 Human
- Unigene: 194148 Human
Anti-Yes1 (phospho Y537) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Yes1 antibody (ab60028)
Formalin fixed and paraffin embedded human hepatocarcinoma tissue reacted with ab60028 at a dilution of 1/50, which was peroxidase conjugated to the secondary antibody, followed by AEC staining.
Western blot - Yes1 (phospho Y537) antibody (ab60028)
Lane 1 : Anti-Yes1 (phospho Y537) antibody (ab60028) at 2.5 µg/ml
Lane 2 : Anti-Yes1 (phospho Y537) antibody (ab60028) at 1 µg/ml
Lane 3 : Anti-Yes1 (phospho Y537) antibody (ab60028) at 0.62 µg/ml
Lane 1 : Cell lysate prepared from human Jurkat cells
Lane 2 : Cell l;ysate prepared from human Jurkat cells
Lane 3 : Cell lysate prepared from human Jurkat cells
Lysates/proteins at 40 µg per lane.
developed using the ECL technique
Predicted band size : 61 kDa
References for Anti-Yes1 (phospho Y537) antibody (ab60028)
ab60028 has not yet been referenced specifically in any publications.
Publishing research using ab60028? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"