Overview

  • Product name
    Z-IETD-FMK
  • Description
    Caspase-8 inhibitor
  • Biological description
    Caspase-8 inhibitor. Inhibits T cell proliferation. Protects against oxyhemoglobin-induced cell death in vitro. Immunosuppressive agent. Active in vitro.
  • Purity
    > 99%

Properties

Applications

Our Abpromise guarantee covers the use of ab141382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab13847 staining active caspase 3 in SKNSH cells treated with Z-IETD-FMK (ab141382), by ICC/IF. Decrease in active caspase 3 expression correlates with increased concentration of Z-IETD-FMK, as described in literature.
    The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141382 (Z-IETD-FMK) in DMSO. After this incupation 10 µM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24 hours. The samples were then fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab13847 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Lawrence CP & Chow SC Suppression of human T cell proliferation by the caspase inhibitors, z-VAD-FMK and z-IETD-FMK is independent of their caspase inhibition properties. Toxicol Appl Pharmacol 265:103-12 (2012). Read more (PubMed: 22982538) »
  • Meguro T  et al. Oxyhemoglobin induces caspase-mediated cell death in cerebral endothelial cells. J Neurochem 77:1128-35 (2001). Read more (PubMed: 11359878) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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