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Anti-ZAP70 antibody [E267] (ab32410)

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Overview

Product name

Anti-ZAP70 antibody [E267]
See all ZAP70 products (29) ...

Description

Rabbit monoclonal [E267] to ZAP70

Specificity

ab32410 recognises Zap-70, a Syk-family protein tyrosine kinase.

Tested applications

Flow Cyt, WB, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues in N terminus of ZAP70 was used as immunogen (Human)

Positive control

Jurkat cell lysate

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

E267

Isotype

IgG

Research areas

Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD

  • Western blot - ZAP70 antibody [E267] (ab32410)Western blot - ZAP70 antibody [E267] (ab32410) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Flow Cyt

     Flow Cyt: 1/40.

    Flow Cyt: 1/40.

  • ShowHide1 Image

    WB

     WB: 1/500. Predicted mol...Read more →

    WB: 1/500. Predicted molecular weight: 70 kDa.

  • IP

     IP: 1/30.

    IP: 1/30.

Application notes

Is unsuitable for ,ICC or IHC.

Target

Function

Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

Tissue specificity

Expressed in T- and natural killer cells.

Involvement in disease

Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.

Domain

The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.

Post-translational
modifications

Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.

Cellular localization

Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.

Target information above from: UniProt accessionP43403 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 70 kDa zeta associated protein antibody
  • 70 kDa zeta-associated protein antibody
  • EC 2.7.10.2 antibody
  • FLJ17670 antibody
  • FLJ17679 antibody
  • Selective T cell defect antibody
  • SRK antibody
  • STD antibody
  • Syk related tyrosine kinase antibody
  • Syk-related tyrosine kinase antibody
  • Truncated ZAP kinase antibody
  • Tyrosine protein kinase ZAP70 antibody
  • Tyrosine-protein kinase ZAP-70 antibody
  • TZK antibody
  • ZAP 70 antibody
  • ZAP-70 antibody
  • ZAP70 antibody
  • ZAP70_HUMAN antibody
  • Zeta chain associated protein kinase 70kD antibody
  • Zeta chain associated protein kinase 70kDa antibody
  • Zeta chain associated protein kinase 70kDa isoform 1 antibody
  • Zeta chain associated protein kinase 70kDa isoform 2 antibody
  • Zeta chain TCR associated protein kinase 70kD antibody
  • Zeta chain TCR associated protein kinase 70kDa antibody
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Database links

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Anti-ZAP70 antibody [E267] images:

  Western blot - ZAP70 antibody [E267] (ab32410)

Western blot - ZAP70 antibody [E267] (ab32410)

Anti-ZAP70 antibody [E267] (ab32410) at 1/500 dilution + Jurkat cell lysates

Predicted band size : 70 kDa
Observed band size : 70 kDa


Western blot analysis on Jurkat Cell lysate.

References for Anti-ZAP70 antibody [E267] (ab32410)

ab32410 has not yet been referenced specifically in any publications.

Publishing research using ab32410? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"