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Western blot - ZAP70 antibody [YE291] (ab32429)
![Western blot - ZAP70 antibody [YE291] (ab32429)](/ps/datasheet/images/ab32429-1.JPG)
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Immunohistochemistry (Paraffin-embedded sections) - ZAP70 antibody [YE291] (ab32429)
![Immunohistochemistry (Paraffin-embedded sections) - ZAP70 antibody [YE291] (ab32429)](/ps/datasheet/images/ab32429-2.JPG)
(enlarge)
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Product Name
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ZAP70 antibody [YE291]
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See all ZAP70 antibodies (25)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [YE291] to ZAP70
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Immunogen
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A synthetic peptide corresponding to residues in C terminus of human ZAP70.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/80.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/500. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: Jurkat cell lysate.
IHC-P: Human lymph node tissue.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
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Relevance
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ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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YE291
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Isotype
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IgG
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZAP70 antibody [YE291] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ZAP70 antibody [YE291] images, references, products related to ab32429 and other tools.
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ZAP70 antibody [YE291] images:
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 Western blot - ZAP70 antibody [YE291] (ab32429)
ZAP70 antibody [YE291] (ab32429) at 1/500 dilution + Jurkat cell lysate
Predicted band size : 70 kDa
Observed band size : 70 kDa
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Immunohistochemistry (Paraffin-embedded sections) - ZAP70 antibody [YE291] (ab32429)
Ab32429, at a 1/100 dilution, staining ZAP70 in paraffin embedded human lymph node tissue by immunohistochemistry.
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Search PubMed (MEDLINE) for references to ZAP70
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ZAP70 antibody [YE291] - more information
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Customer reviews (feedback) regarding ZAP70 antibody [YE291] |
Customer FAQs regarding ZAP70 antibody [YE291] |
Protocols for ZAP70 antibody [YE291] |
Price and availability of products related to ZAP70 antibody [YE291] |
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ZAP70 antibody [YE291] - related products:
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ZAP70 antibody [YE291] - other tools:
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Contact Abcam with a Technical Enquiry about ZAP70 antibody [YE291] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - ZAP70 antibody [YE291] (ab32429)](/index.html?datasheet=32429#ab32429-1.JPG)
![Immunohistochemistry (Paraffin-embedded sections) - ZAP70 antibody [YE291] (ab32429)](/index.html?datasheet=32429#ab32429-2.JPG)
