You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-ZAP70 antibody [YE291] (ab32429)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-ZAP70 antibody [YE291]
See all ZAP70 products (28) ...

Description

Rabbit monoclonal [YE291] to ZAP70

Tested applications

WB, IHC-P, ICC, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in C terminus of human ZAP70.

Positive control

WB: Jurkat cell lysate. IHC-P: Human lymph node tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

YE291

Isotype

IgG

Research areas

Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD

  • Western blot - ZAP70 antibody [YE291] (ab32429)Western blot - ZAP70 antibody [YE291] (ab32429) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - ZAP70 antibody [YE291] (ab32429)Immunohistochemistry (Paraffin-embedded sections) - ZAP70 antibody [YE291] (ab32429) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500Detects a band ...Read more →

    WB: 1/500Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.

    IHC-P: 1/100 - 1/250.

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

  • Flow Cyt

     Flow Cyt: 1/80

    Flow Cyt: 1/80

  • IP

     IP: 1/50

    IP: 1/50

Target

Function

Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

Tissue specificity

Expressed in T- and natural killer cells.

Involvement in disease

Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.

Domain

The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.

Post-translational
modifications

Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.

Cellular localization

Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.

Target information above from: UniProt accessionP43403 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 70 kDa zeta associated protein antibody
  • 70 kDa zeta-associated protein antibody
  • EC 2.7.10.2 antibody
  • FLJ17670 antibody
  • FLJ17679 antibody
  • Selective T cell defect antibody
  • SRK antibody
  • STD antibody
  • Syk related tyrosine kinase antibody
  • Syk-related tyrosine kinase antibody
  • Truncated ZAP kinase antibody
  • Tyrosine protein kinase ZAP70 antibody
  • Tyrosine-protein kinase ZAP-70 antibody
  • TZK antibody
  • ZAP 70 antibody
  • ZAP-70 antibody
  • ZAP70 antibody
  • ZAP70_HUMAN antibody
  • Zeta chain associated protein kinase 70kD antibody
  • Zeta chain associated protein kinase 70kDa antibody
  • Zeta chain associated protein kinase 70kDa isoform 1 antibody
  • Zeta chain associated protein kinase 70kDa isoform 2 antibody
  • Zeta chain TCR associated protein kinase 70kD antibody
  • Zeta chain TCR associated protein kinase 70kDa antibody
see all

Database links

Anti-ZAP70 antibody [YE291] images:

  Western blot - ZAP70 antibody [YE291] (ab32429)

Western blot - ZAP70 antibody [YE291] (ab32429)

Anti-ZAP70 antibody [YE291] (ab32429) at 1/500 dilution + Jurkat cell lysate

Predicted band size : 70 kDa
Observed band size : 70 kDa

  Immunohistochemistry (Paraffin-embedded sections) - ZAP70 antibody [YE291] (ab32429)

Immunohistochemistry (Paraffin-embedded sections) - ZAP70 antibody [YE291] (ab32429)

Ab32429, at a 1/100 dilution, staining ZAP70 in paraffin embedded human lymph node tissue by immunohistochemistry.

References for Anti-ZAP70 antibody [YE291] (ab32429)

ab32429 has not yet been referenced specifically in any publications.

Publishing research using ab32429? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"