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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab38871 for help.
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![Flow Cytometry / FACS - ZAP70 antibody [ZAP-03] (ab38871)](/ps/datasheet/Images/38/ab38871/38871_1.bmp)
Flow Cytometry / FACS - ZAP70 antibody [ZAP-03] (ab38871)
Flow Cytometry analysis of the human peripheral blood of a patient with B-cell chronic lymphatic leukemia (B-CLL) using antibody ab38871; Dot Plot diagram shows the distribution of lymphocyte populations: T-lymphocytes (green), B-lymphocytes (blue) and NK cells (red).
![Western blot - ZAP70 antibody [ZAP-03] (ab38871)](/ps/datasheet/Images/38/ab38871/38871_2.bmp)
Western blot - ZAP70 antibody [ZAP-03] (ab38871)
Lane 1 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 4 µg/ml
Lane 2 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 2 µg/ml
Lane 3 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 1 µg/ml
Lane 4 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
Lane 5 : ZAP-70 specific antibody 1E7.2 at 1 µg/ml
Lane 6 : Isotypic control PPV-06 (mouse IgG1).
Lane 1 : HPB-ALL peripheral blood leukemia T-cell line
Lane 2 : HPB-ALL peripheral blood leukemia T-cell line
Lane 3 : HPB-ALL peripheral blood leukemia T-cell line
Lane 4 : HPB-ALL peripheral blood leukemia T-cell line
Lane 5 : HPB-ALL peripheral blood leukemia T-cell line
Lane 6 : HPB-ALL peripheral blood leukemia T-cell line
Performed under reducing conditions.
Predicted band size : 70 kDa
Observed band size : 70 kDa
Additional bands at : 55 kDa (possible IgG,degradation product).
Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line; SDS-PAGE; 10% separating gel, 4% stacking gel.
ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
![Western blot - ZAP70 antibody [ZAP-03] (ab38871)](/ps/datasheet/Images/38/ab38871/38871_3.bmp)
Western blot - ZAP70 antibody [ZAP-03] (ab38871)
Lanes 1 - 2 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
Lanes 3 - 4 : Isotypic control PPV-06 (mouse IgG1)
Lane 1 : HPB-ALL cell line
Lane 2 : RAMOS cell line
Lane 3 : HPB-ALL cell line
Lane 4 : RAMOS cell line
Performed under reducing conditions.
Predicted band size : 70 kDa
Observed band size : 70 kDa
Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line and RAMOS human Burkitt lymphoma cell line; SDS-PAGE; 10% separating gel, 4% stacking gel.
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