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Flow Cytometry / FACS - ZAP70 antibody [ZAP-03] (ab38871)
![Flow Cytometry / FACS - ZAP70 antibody [ZAP-03] (ab38871)](/ps/datasheet/images/38871_1.bmp)
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Western blot - ZAP70 antibody [ZAP-03] (ab38871)
![Western blot - ZAP70 antibody [ZAP-03] (ab38871)](/ps/datasheet/images/38871_2.bmp)
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Western blot - ZAP70 antibody [ZAP-03] (ab38871)
![Western blot - ZAP70 antibody [ZAP-03] (ab38871)](/ps/datasheet/images/38871_3.bmp)
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Product Name
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ZAP70 antibody [ZAP-03]
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See all ZAP70 antibodies (25)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [ZAP-03] to ZAP70
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Immunogen
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Recombinant fragment representing C-terminal part (160 amino acids) of human ZAP-70.
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Reacts with
(species key)
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Hu
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Specificity
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ab38871 recognises ZAP-70, a 70 kDa protein tyrosine kinase expressed in T
and NK cells.
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Tested applications
(see key)
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Flow Cyt, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at a concentration of 2 - 5 µg/ml.
WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HPB-ALL human peripheral blood leukemia T-cell line
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
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Relevance
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ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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ZAP-03
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Isotype
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IgG1
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from hybridoma culture supernatant by protein-A affinity chromatography
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZAP70 antibody [ZAP-03] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ZAP70 antibody [ZAP-03] images, references, products related to ab38871 and other tools.
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ZAP70 antibody [ZAP-03] images:
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 Flow Cytometry / FACS - ZAP70 antibody [ZAP-03] (ab38871)
Flow Cytometry analysis of the human peripheral blood of a patient with B-cell chronic lymphatic leukemia (B-CLL) using antibody ab38871; Dot Plot diagram shows the distribution of lymphocyte populations: T-lymphocytes (green), B-lymphocytes (blue) and NK cells (red).
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Western blot - ZAP70 antibody [ZAP-03] (ab38871)
Lane 1 : ZAP70 antibody [ZAP-03] (ab38871) at 4 µg/ml
Lane 2 : ZAP70 antibody [ZAP-03] (ab38871) at 2 µg/ml
Lane 3 : ZAP70 antibody [ZAP-03] (ab38871) at 1 µg/ml
Lane 4 : ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
Lane 5 : ZAP-70 specific antibody 1E7.2 at 1 µg/ml
Lane 6 : Isotypic control PPV-06 (mouse IgG1).
Lane 1 : HPB-ALL peripheral blood leukemia T-cell line
Lane 2 : HPB-ALL peripheral blood leukemia T-cell line
Lane 3 : HPB-ALL peripheral blood leukemia T-cell line
Lane 4 : HPB-ALL peripheral blood leukemia T-cell line
Lane 5 : HPB-ALL peripheral blood leukemia T-cell line
Lane 6 : HPB-ALL peripheral blood leukemia T-cell line
Performed under reducing conditions.
Predicted band size : 70 kDa
Observed band size : 70 kDa
Additional bands at : 55 kDa (possible IgG,degradation product).
Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line; SDS-PAGE; 10% separating gel, 4% stacking gel.
ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
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Western blot - ZAP70 antibody [ZAP-03] (ab38871)
Lanes 1 - 2 : ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
Lanes 3 - 4 : Isotypic control PPV-06 (mouse IgG1)
Lane 1 : HPB-ALL cell line
Lane 2 : RAMOS cell line
Lane 3 : HPB-ALL cell line
Lane 4 : RAMOS cell line
Performed under reducing conditions.
Predicted band size : 70 kDa
Observed band size : 70 kDa
Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line and RAMOS human Burkitt lymphoma cell line; SDS-PAGE; 10% separating gel, 4% stacking gel.
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Search PubMed (MEDLINE) for references to ZAP70
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ZAP70 antibody [ZAP-03] - more information
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Customer reviews (feedback) regarding ZAP70 antibody [ZAP-03] |
Customer FAQs regarding ZAP70 antibody [ZAP-03] |
Protocols for ZAP70 antibody [ZAP-03] |
Price and availability of products related to ZAP70 antibody [ZAP-03] |
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ZAP70 antibody [ZAP-03] - related products:
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ZAP70 antibody [ZAP-03] - other tools:
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Contact Abcam with a Technical Enquiry about ZAP70 antibody [ZAP-03] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Flow Cytometry / FACS - ZAP70 antibody [ZAP-03] (ab38871)](/index.html?datasheet=38871#38871_1.bmp)
![Western blot - ZAP70 antibody [ZAP-03] (ab38871)](/index.html?datasheet=38871#38871_2.bmp)
![Western blot - ZAP70 antibody [ZAP-03] (ab38871)](/index.html?datasheet=38871#38871_3.bmp)
