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Anti-ZAP70 antibody [ZAP-03] (ab38871)

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Overview

Product name

Anti-ZAP70 antibody [ZAP-03]
See all ZAP70 products (28) ...

Description

Mouse monoclonal [ZAP-03] to ZAP70

Specificity

ab38871 recognises ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells.

Tested applications

Flow Cyt, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment representing C-terminal part (160 amino acids) of human ZAP-70.

Positive control

HPB-ALL human peripheral blood leukemia T-cell line

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

>95% by SDS-PAGE

Purification notes

Purified from hybridoma culture supernatant by protein-A affinity chromatography

Clonality

Monoclonal

Clone number

ZAP-03

Isotype

IgG1

Research areas

Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD

  • Flow Cytometry / FACS - ZAP70 antibody [ZAP-03] (ab38871)Flow Cytometry / FACS - ZAP70 antibody [ZAP-03] (ab38871) image (enlarge)

  • Western blot - ZAP70 antibody [ZAP-03] (ab38871)Western blot - ZAP70 antibody [ZAP-03] (ab38871) image (enlarge)

  • Western blot - ZAP70 antibody [ZAP-03] (ab38871)Western blot - ZAP70 antibody [ZAP-03] (ab38871) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab38871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

Tissue specificity

Expressed in T- and natural killer cells.

Involvement in disease

Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.

Domain

The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.

Post-translational
modifications

Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.

Cellular localization

Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.

Target information above from: UniProt accessionP43403 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 70 kDa zeta associated protein antibody
  • 70 kDa zeta-associated protein antibody
  • EC 2.7.10.2 antibody
  • FLJ17670 antibody
  • FLJ17679 antibody
  • Selective T cell defect antibody
  • SRK antibody
  • STD antibody
  • Syk related tyrosine kinase antibody
  • Syk-related tyrosine kinase antibody
  • Truncated ZAP kinase antibody
  • Tyrosine protein kinase ZAP70 antibody
  • Tyrosine-protein kinase ZAP-70 antibody
  • TZK antibody
  • ZAP 70 antibody
  • ZAP-70 antibody
  • ZAP70 antibody
  • ZAP70_HUMAN antibody
  • Zeta chain associated protein kinase 70kD antibody
  • Zeta chain associated protein kinase 70kDa antibody
  • Zeta chain associated protein kinase 70kDa isoform 1 antibody
  • Zeta chain associated protein kinase 70kDa isoform 2 antibody
  • Zeta chain TCR associated protein kinase 70kD antibody
  • Zeta chain TCR associated protein kinase 70kDa antibody
see all

Database links

Anti-ZAP70 antibody [ZAP-03] images:

  Flow Cytometry / FACS - ZAP70 antibody [ZAP-03] (ab38871)

Flow Cytometry / FACS - ZAP70 antibody [ZAP-03] (ab38871)

Flow Cytometry analysis of the human peripheral blood of a patient with B-cell chronic lymphatic leukemia (B-CLL) using antibody ab38871; Dot Plot diagram shows the distribution of lymphocyte populations: T-lymphocytes (green), B-lymphocytes (blue) and NK cells (red).

  Western blot - ZAP70 antibody [ZAP-03] (ab38871)

Western blot - ZAP70 antibody [ZAP-03] (ab38871)

Lane 1 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 4 µg/ml
Lane 2 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 2 µg/ml
Lane 3 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 1 µg/ml
Lane 4 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
Lane 5 : ZAP-70 specific antibody 1E7.2 at 1 µg/ml
Lane 6 : Isotypic control PPV-06 (mouse IgG1).

Lane 1 : HPB-ALL peripheral blood leukemia T-cell line
Lane 2 : HPB-ALL peripheral blood leukemia T-cell line
Lane 3 : HPB-ALL peripheral blood leukemia T-cell line
Lane 4 : HPB-ALL peripheral blood leukemia T-cell line
Lane 5 : HPB-ALL peripheral blood leukemia T-cell line
Lane 6 : HPB-ALL peripheral blood leukemia T-cell line


Performed under reducing conditions.

Predicted band size : 70 kDa
Observed band size : 70 kDa
Additional bands at : 55 kDa (possible IgG,degradation product).

Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line; SDS-PAGE; 10% separating gel, 4% stacking gel. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.

  Western blot - ZAP70 antibody [ZAP-03] (ab38871)

Western blot - ZAP70 antibody [ZAP-03] (ab38871)

Lanes 1 - 2 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
Lanes 3 - 4 : Isotypic control PPV-06 (mouse IgG1)

Lane 1 : HPB-ALL cell line
Lane 2 : RAMOS cell line
Lane 3 : HPB-ALL cell line
Lane 4 : RAMOS cell line


Performed under reducing conditions.

Predicted band size : 70 kDa
Observed band size : 70 kDa


Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line and RAMOS human Burkitt lymphoma cell line; SDS-PAGE; 10% separating gel, 4% stacking gel.

References for Anti-ZAP70 antibody [ZAP-03] (ab38871)

ab38871 has not yet been referenced specifically in any publications.

Publishing research using ab38871? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"