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Western blot - ZAP70 antibody (ab13957)
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Product Name
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ZAP70 antibody
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See all ZAP70 antibodies (25)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ZAP70
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu, Ms
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Specificity
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This antibody detects ZAP70 only.
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: 1/50.
WB: 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat (Human T cell lymphoblast-like cell line).
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
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Relevance
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ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 50% Glycerol, PBS Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Purification notes
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This antibody is affinity purified.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Add antibacterial agent such as sodium azide.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZAP70 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ZAP70 antibody images, references, products related to ab13957 and other tools.
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ZAP70 antibody images:
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 Western blot - ZAP70 antibody (ab13957)
All lanes : ZAP70 antibody (ab13957) at 1/500 dilution
Lane 1 : Molecular Weight Marker
Lane 2 : Jurkat whole cell lysate (20 µg)
Lane 3 : Jurkat whole cell lysate (20 µg)
Secondary
IR680 Goat anti-rabbits IgG conjugate at 1/10000 dilution
Predicted band size : 70 kDa
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Search PubMed (MEDLINE) for references to ZAP70
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ZAP70 antibody - more information
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Customer reviews (feedback) regarding ZAP70 antibody |
Customer FAQs regarding ZAP70 antibody |
Protocols for ZAP70 antibody |
Price and availability of products related to ZAP70 antibody |
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ZAP70 antibody - related products:
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ZAP70 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ZAP70 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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