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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-ZAP70 antibody
See all ZAP70 products (28) ...
Description
Rabbit polyclonal to ZAP70
Specificity
This antibody detects ZAP70 only.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant full length protein (Human).
Positive control
Jurkat (Human T cell lymphoblast-like cell line).
General notes
Add antibacterial agent such as sodium azide.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
This antibody is affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
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Western blot - ZAP70 antibody (ab13957)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP
IP: 1/50IP: 1/50
Target
Function
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Tissue specificity
Expressed in T- and natural killer cells.
Involvement in disease
Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.
Domain
The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
Post-translational
modifications
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Cellular localization
Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
Target information above from: UniProt accessionP43403
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 2.7.10.2 antibody
see all
Database links
- Entrez Gene: 7535 Human
- Entrez Gene: 22637 Mouse
- Omim: 176947 Human
- SwissProt: P43403 Human
- SwissProt: P43404 Mouse
- Unigene: 234569 Human
- Unigene: 8038 Mouse
Anti-ZAP70 antibody images:
Western blot - ZAP70 antibody (ab13957)
All lanes : Anti-ZAP70 antibody (ab13957) at 1/500 dilution
Lane 1 : Molecular Weight Marker
Lane 2 : Jurkat whole cell lysate (20 µg)
Lane 3 : Jurkat whole cell lysate (20 µg)
Secondary
IR680 Goat anti-rabbits IgG conjugate at 1/10000 dilution
Predicted band size : 70 kDa
References for Anti-ZAP70 antibody (ab13957)
ab13957 has not yet been referenced specifically in any publications.
Publishing research using ab13957? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"