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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-ZAP70 antibody, prediluted
See all ZAP70 products (28) ...
Description
Rabbit polyclonal to ZAP70, prediluted
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to C terminal of human ZAP70.
Positive control
Jurkat cells. Tonsil tissue.
Properties
Form
Prediluted
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 50mM Tris, pH 7.6
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.(use neat for 30 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min and then cool at room temperature for 20 min.)
WB: Use at an assay dependent dilution. Predicted molecular weight: 70 kDa.
Target
Function
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Tissue specificity
Expressed in T- and natural killer cells.
Involvement in disease
Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.
Domain
The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
Post-translational
modifications
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Cellular localization
Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
Target information above from: UniProt accessionP43403
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 2.7.10.2 antibody
see all
Database links
- Entrez Gene: 7535 Human
- Omim: 176947 Human
- SwissProt: P43403 Human
- Unigene: 234569 Human
Anti-ZAP70 antibody, prediluted images:
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - ZAP70 antibody (ab31009)
Immunohistochemical analysis of ZAP70 expression in paraffin embedded formalin fixed human tonsil tissue section, using neat ab31009.
Western blot - ZAP70 antibody, prediluted (ab31009)
Anti-ZAP70 antibody, prediluted (ab31009) at 1/25 dilution + NIH3T3 cell lysate
Predicted band size : 70 kDa
Observed band size : 70 kDa
The dilution given for this image is the dilution that was used for a non-prediluted version of this antibody.
References for Anti-ZAP70 antibody, prediluted (ab31009)
ab31009 has not yet been referenced specifically in any publications.
Publishing research using ab31009? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"