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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](/ps/datasheet/Images/76/ab76501/ab76501.JPG)
Western blot - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
All lanes : Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501) at 1/10000 dilution
Lane 1 : Jurkat cell lysate (untreated)
Lane 2 : Jurkat cell lysate, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
goat anit-rabbit HRP labeled IgG at 1/2000 dilution
Predicted band size : 70 kDa
Observed band size : 70 kDa
![Flow Cytometry - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](/ps/datasheet/Images/76/ab76501/ab76501 FC.JPG)
Flow Cytometry - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
Flow cytometric analysis of permeabilized Jurkat cells, untreated (green), pervanadate treated (red) stained with ab76501, at 1/20 dilution (blue) and pervanadate-treated Jurkat cells using the same antibody preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho- ZAP70 (Tyr 292)).
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