![Western blot - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501) image](#ab76501.JPG)
![Flow Cytometry - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501) image](#ab76501 FC.JPG)
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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-ZAP70 (phospho Y292) antibody [EPR1073]
See all ZAP70 products (28) ...
Description
Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292)
Specificity
ab76501 only detects ZAP70 when phosphorylated at Tyrosine 292.
Tested applications
ICC, WB, Flow Cytmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic phospho-specific peptide corresponding to residues surrounding Tyrosine 292 of human ZAP70
Positive control
Jurkat cell lysate, untreated or treated with pervanadate
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR1073
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
Flow Cyt: 1/20.
ICC: 1/250 - 1/500.
WB: 1/5000 - 1/10000. Predicted molecular weight: 70 kDa.
Is unsuitable for IHC and IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Tissue specificity
Expressed in T- and natural killer cells.
Involvement in disease
Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.
Domain
The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
Post-translational
modifications
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Cellular localization
Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
Target information above from: UniProt accessionP43403
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 2.7.10.2 antibody
see all
Database links
- Entrez Gene: 7535 Human
- Omim: 176947 Human
- SwissProt: P43403 Human
- Unigene: 234569 Human
Anti-ZAP70 (phospho Y292) antibody [EPR1073] images:
Western blot - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
![Western blot - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](/ps/datasheet/Images/76/ab76501/ab76501.JPG)
All lanes : Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501) at 1/10000 dilution
Lane 1 : Jurkat cell lysate (untreated)
Lane 2 : Jurkat cell lysate, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
goat anit-rabbit HRP labeled IgG at 1/2000 dilution
Predicted band size : 70 kDa
Observed band size : 70 kDa
Flow Cytometry - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
![Flow Cytometry - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](/ps/datasheet/Images/76/ab76501/ab76501 FC.JPG)
Flow cytometric analysis of permeabilized Jurkat cells, untreated (green), pervanadate treated (red) stained with ab76501, at 1/20 dilution (blue) and pervanadate-treated Jurkat cells using the same antibody preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho- ZAP70 (Tyr 292)).
References for Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
ab76501 has not yet been referenced specifically in any publications.
Publishing research using ab76501? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"