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Western blot - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
![Western blot - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](/ps/datasheet/images/ab76501.JPG)
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Flow Cytometry - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
![Flow Cytometry - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](/ps/datasheet/images/ab76501 FC.JPG)
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Product Name
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ZAP70 (phospho Y292) antibody [EPR1073]
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See all ZAP70 antibodies (25)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292)
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Immunogen
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Synthetic phospho-specific peptide corresponding to residues surrounding Tyrosine 292 of human ZAP70
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Specificity
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ab76501 only detects ZAP70 when phosphorylated at Tyrosine 292.
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Tested applications
(see key)
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Flow Cyt, ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/20.
ICC: 1/250 - 1/500.
WB: 1/5000 - 1/10000. Predicted molecular weight: 70 kDa.
Is unsuitable for IHC and IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate, untreated or treated with pervanadate
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
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Relevance
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ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.
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ZAP70 (phospho Y292) antibody [EPR1073] (ab76501) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EPR1073
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZAP70 (phospho Y292) antibody [EPR1073] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ZAP70 (phospho Y292) antibody [EPR1073] images, references, products related to ab76501 and other tools.
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ZAP70 (phospho Y292) antibody [EPR1073] images:
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 Western blot - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
All lanes : ZAP70 (phospho Y292) antibody [EPR1073] (ab76501) at 1/10000 dilution
Lane 1 : Jurkat cell lysate (untreated)
Lane 2 : Jurkat cell lysate, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
goat anit-rabbit HRP labeled IgG at 1/2000 dilution
Predicted band size : 70 kDa
Observed band size : 70 kDa
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Flow Cytometry - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
Flow cytometric analysis of permeabilized Jurkat cells, untreated (green), pervanadate treated (red) stained with ab76501, at 1/20 dilution (blue) and pervanadate-treated Jurkat cells using the same antibody
preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho-
ZAP70 (Tyr 292)).
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Search PubMed (MEDLINE) for references to ZAP70
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ZAP70 (phospho Y292) antibody [EPR1073] - more information
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Customer reviews (feedback) regarding ZAP70 (phospho Y292) antibody [EPR1073] |
Customer FAQs regarding ZAP70 (phospho Y292) antibody [EPR1073] |
Protocols for ZAP70 (phospho Y292) antibody [EPR1073] |
Price and availability of products related to ZAP70 (phospho Y292) antibody [EPR1073] |
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ZAP70 (phospho Y292) antibody [EPR1073] - related products:
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ZAP70 (phospho Y292) antibody [EPR1073] - other tools:
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Contact Abcam with a Technical Enquiry about ZAP70 (phospho Y292) antibody [EPR1073] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](/index.html?datasheet=76501#ab76501.JPG)
![Flow Cytometry - ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](/index.html?datasheet=76501#ab76501 FC.JPG)
