|
|
|
|
Western blot - ZAP70 (phospho Y292) antibody (ab12868)
(enlarge)
|
|
|
|
|
Product Name
|
ZAP70 (phospho Y292) antibody
|
| |
See all ZAP70 antibodies (25)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to ZAP70 (phospho Y292)
|
|
Immunogen
|
Synthetic peptide derived from a region of human ZAP70 that contains tyrosine 292 (unfortunately, the amino acid sequence is considered to be commercially sensitive)
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Ms
(Due to sequence homology)
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: 1/1000. Predicted molecular weight: 74.3 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Jurkat cells treated with hydrogen peroxide.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
|
|
Relevance
|
ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.
|
|
|
ZAP70 (phospho Y292) antibody (ab12868) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: Dulbecco's PBS without magnesium and calcium, 50% Glycerol, PBS, 1mg/ml BSA, pH 7.3 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Purification notes
|
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ZAP70. The final product is generated by affinity chromatography using a ZAP70-derived peptide that is phosphorylated at threonine 292.
|
|
Form
|
Liquid
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
|
Notes
|
Centrifuge before opening to settle contents.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZAP70 (phospho Y292) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for ZAP70 (phospho Y292) antibody images, references, products related to ab12868 and other tools.
|
| |
ZAP70 (phospho Y292) antibody images:
|
 Western blot - ZAP70 (phospho Y292) antibody (ab12868)
Predicted band size : 74.3 kDa
Peptide Competition and Phosphatase Treatment: Lysates prepared from Jurkat cells left untreated (1) or treated with hydrogen peroxide (2-6) were either left untreated (1-5) or treated with lambda phosphatase (6). Lysates were then incubated with ab12868 following prior incubation with: no peptide (1, 6), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine-containing peptide (4) or, the phosphopeptide immunogen (5). The data show that only the peptide corresponding to ZAP70 [phospho Y292] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
|
| |
| |
|
Search PubMed (MEDLINE) for references to ZAP70
|
| |
ZAP70 (phospho Y292) antibody - more information
|
Customer reviews (feedback) regarding ZAP70 (phospho Y292) antibody |
Customer FAQs regarding ZAP70 (phospho Y292) antibody |
Protocols for ZAP70 (phospho Y292) antibody |
Price and availability of products related to ZAP70 (phospho Y292) antibody |
| |
ZAP70 (phospho Y292) antibody - related products:
|
|
|
| |
ZAP70 (phospho Y292) antibody - other tools:
|
Contact Abcam with a Technical Enquiry about ZAP70 (phospho Y292) antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
