|
|
|
|
Western blot - ZAP70 (phospho Y292) antibody (ab59290)
(enlarge)
|
|
|
|
|
Product Name
|
ZAP70 (phospho Y292) antibody
|
| |
See all ZAP70 antibodies (25)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to ZAP70 (phospho Y292)
|
|
Immunogen
|
Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of tyrosine 292 (D-G-YP-T-P).
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Specificity
|
Detects endogenous levels of ZAP-70 only when phosphorylated at tyrosine 292.
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
UV treated Jurkat cells.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
|
|
Relevance
|
ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZAP70 (phospho Y292) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for ZAP70 (phospho Y292) antibody images, references, products related to ab59290 and other tools.
|
| |
ZAP70 (phospho Y292) antibody images:
|
 Western blot - ZAP70 (phospho Y292) antibody (ab59290)
All lanes : ZAP70 (phospho Y292) antibody (ab59290) at 1/500 dilution
Lane 1 : extracts from Jurkat cells, treated with UV (15mins)
Lane 2 : extracts from Jurkat cells, treated with UV (15mins) with immunizing peptide
Predicted band size : 70 kDa
Observed band size : 70 kDa
|
| |
| |
|
Search PubMed (MEDLINE) for references to ZAP70
|
| |
ZAP70 (phospho Y292) antibody - more information
|
Customer reviews (feedback) regarding ZAP70 (phospho Y292) antibody |
Customer FAQs regarding ZAP70 (phospho Y292) antibody |
Protocols for ZAP70 (phospho Y292) antibody |
Price and availability of products related to ZAP70 (phospho Y292) antibody |
| |
ZAP70 (phospho Y292) antibody - related products:
|
|
|
| |
ZAP70 (phospho Y292) antibody - other tools:
|
Contact Abcam with a Technical Enquiry about ZAP70 (phospho Y292) antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
