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Western blot - ZAP70 (phospho Y292) antibody (ab59290)
Western blot - ZAP70 (phospho Y292) antibody (ab59290)
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Product Name 

ZAP70 (phospho Y292) antibody

 

See all ZAP70 antibodies (25)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ZAP70 (phospho Y292)

Immunogen 

Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of tyrosine 292 (D-G-YP-T-P).

Reacts with 
(species key)

Hu, Ms

Specificity 

Detects endogenous levels of ZAP-70 only when phosphorylated at tyrosine 292.

Tested applications 
(see key)

ELISA, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

UV treated Jurkat cells.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD

Relevance 

ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.

 

Alternative names ZAP70 (phospho Y292) antibody (ab59290)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

7535    (Human)
22637    (Mouse)

Omim

    

176947    (Human)

SwissProt

    

P43403    (Human)
P43404    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZAP70 (phospho Y292) antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ZAP70 (phospho Y292) antibody images, references, products related to ab59290 and other tools.

 

ZAP70 (phospho Y292) antibody images:

Product image  Western blot - ZAP70 (phospho Y292) antibody (ab59290)

Western blot - ZAP70 (phospho Y292) antibody (ab59290)

All lanes : ZAP70 (phospho Y292) antibody (ab59290) at 1/500 dilution

Lane 1 : extracts from Jurkat cells, treated with UV (15mins)
Lane 2 : extracts from Jurkat cells, treated with UV (15mins) with immunizing peptide


Predicted band size : 70 kDa
Observed band size : 70 kDa

 
 

Search PubMed (MEDLINE) for references to ZAP70

 

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