Loading...
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Anti-ZAP70 (phospho Y292) antibody
See all ZAP70 products (28) ...
Rabbit polyclonal to ZAP70 (phospho Y292)
Detects endogenous levels of ZAP-70 only when phosphorylated at tyrosine 292.
ELISA, WBmore details
Reacts with
Human
Predicted to work with
Mouse
Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of tyrosine 292 (D-G-YP-T-P).
UV treated Jurkat cells.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
Western blot - ZAP70 (phospho Y292) antibody (ab59290)
(enlarge)
Our Abpromise guarantee covers the use of ab59290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/5000
WB: 1/500 - 1/1000.Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Expressed in T- and natural killer cells.
Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.
The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
Target information above from: UniProt accessionP43403
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - ZAP70 (phospho Y292) antibody (ab59290)

All lanes : Anti-ZAP70 (phospho Y292) antibody (ab59290) at 1/500 dilution
Lane 1 : extracts from Jurkat cells, treated with UV (15mins)
Lane 2 : extracts from Jurkat cells, treated with UV (15mins) with immunizing peptide
Predicted band size : 70 kDa
Observed band size : 70 kDa
ab59290 has not yet been referenced specifically in any publications.
Publishing research using ab59290? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

All lanes : Anti-ZAP70 (phospho Y292) antibody (ab59290) at 1/500 dilution
Lane 1 : extracts from Jurkat cells, treated with UV (15mins)
Lane 2 : extracts from Jurkat cells, treated with UV (15mins) with immunizing peptide
Predicted band size : 70 kDa
Observed band size : 70 kDa
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Call 01223 696 000 or contact us
