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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Anti-ZAP70 (phospho Y315 + Y319) antibody (ab12869)
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Secondary antibodies
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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab12869? Please let us know so that we can cite the reference in this datasheet
ab12869 has been referenced in 2 publications.
- Martín-Cófreces NB et al. MTOC translocation modulates IS formation and controls sustained T cell signaling. J Cell Biol 182:951-62 (2008). WB; Human. PubMed: 18779373
- Olazabal IM et al. Activation Outcomes Induced in Naive CD8 T Cells by Macrophages Primed via "Phagocytic" and Non-"Phagocytic" Pathways. Mol Biol Cell : (2007). WB; Mouse. PubMed: 18077558
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Anti-ZAP70 (phospho Y315 + Y319) antibody (ab12869)
Predicted band size : 74.3 kDa
Lysates prepared from Jurkat cells left untreated (1) or treated with hydrogen peroxide (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-6) or treated with YOP phosphatase (7), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab12869 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine-containing peptide (4) the phosphopeptide immunogen (5) or, the phosphopeptide corresponding to ZAP70 [phospho Y315] (6). After washing, membranes were incubated with goat IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to ZAP70 [phospho Y315 & phospho Y319] completely blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
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