Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-ZAP70 (phospho Y315 + Y319) antibody
See all ZAP70 products (28) ...
Description
Rabbit polyclonal to ZAP70 (phospho Y315 + Y319)
Specificity
Syk (85% homologous) may cross react in some cell systems.
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from the region of human ZAP70 that contains tyrosines 315 and 319 (unfortunately, the amino acid sequence is considered to be commercially sensitive)
Positive control
Jurkat cells treated with hydrogen peroxide.
General notes
Centrifuge before opening to settle contents.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Dulbecco's PBS without magnesium and calcium, 50% Glycerol, PBS, 1mg/ml BSA, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ZAP70. The final product is generated by affinity chromatography using a ZAP70-derived peptide that is phosphorylated at tyrosines 315 and 319.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
-
Western blot - Anti-ZAP70 (phospho Y315 + Y319) antibody (ab12869)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 74.3 kDa.
Target
Function
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Tissue specificity
Expressed in T- and natural killer cells.
Involvement in disease
Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.
Domain
The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
Post-translational
modifications
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Cellular localization
Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
Target information above from: UniProt accessionP43403
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 2.7.10.2 antibody
see all
Database links
- Entrez Gene: 7535 Human
- Entrez Gene: 22637 Mouse
- Omim: 176947 Human
- SwissProt: P43403 Human
- SwissProt: P43404 Mouse
- Unigene: 234569 Human
- Unigene: 8038 Mouse
Anti-ZAP70 (phospho Y315 + Y319) antibody images:
Western blot - Anti-ZAP70 (phospho Y315 + Y319) antibody (ab12869)
Predicted band size : 74.3 kDa
Lysates prepared from Jurkat cells left untreated (1) or treated with hydrogen peroxide (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-6) or treated with YOP phosphatase (7), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab12869 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine-containing peptide (4) the phosphopeptide immunogen (5) or, the phosphopeptide corresponding to ZAP70 [phospho Y315] (6). After washing, membranes were incubated with goat IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to ZAP70 [phospho Y315 & phospho Y319] completely blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
References for Anti-ZAP70 (phospho Y315 + Y319) antibody (ab12869)
This product has been referenced in:
- Martín-Cófreces NBet al. MTOC translocation modulates IS formation and controls sustained T cell signaling. J Cell Biol 182:951-62 (2008). WB; Human.Read more (PubMed: 18779373) »
- Olazabal IMet al. Activation Outcomes Induced in Naive CD8 T Cells by Macrophages Primed via "Phagocytic" and Non-"Phagocytic" Pathways. Mol Biol Cell : (2007). WB; Mouse.Read more (PubMed: 18077558) »
See all 2 publications for this product
Publishing research using ab12869? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"