|
|
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ZAP70 (phospho Y315) antibody (ab60970)
(enlarge)
|
|
|
|
|
Product Name
|
ZAP70 (phospho Y315) antibody
|
| |
See all ZAP70 antibodies (25)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to ZAP70 (phospho Y315)
|
|
Immunogen
|
Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of tyrosine 315 (S-V-YP-E-S).
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Specificity
|
Detects endogenous levels of ZAP70 only when phosphorylated at tyrosine 315.
|
|
Tested applications
(see key)
|
ELISA, IHC-P
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/5000.
IHC-P: 1/50 - 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Lymph node tissue.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
|
|
Relevance
|
ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Purification notes
|
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at -20°C. Stable for 12 months at -20°C
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZAP70 (phospho Y315) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for ZAP70 (phospho Y315) antibody images, references, products related to ab60970 and other tools.
|
| |
ZAP70 (phospho Y315) antibody images:
|
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ZAP70 (phospho Y315) antibody (ab60970)
ab60970 at 1/50 - 1/100 dilution staining ZAP70 in human lymph node by immunohistochemistry on paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
|
| |
| |
|
Search PubMed (MEDLINE) for references to ZAP70
|
| |
ZAP70 (phospho Y315) antibody - more information
|
Customer reviews (feedback) regarding ZAP70 (phospho Y315) antibody |
Customer FAQs regarding ZAP70 (phospho Y315) antibody |
Protocols for ZAP70 (phospho Y315) antibody |
Price and availability of products related to ZAP70 (phospho Y315) antibody |
| |
ZAP70 (phospho Y315) antibody - related products:
|
|
|
| |
ZAP70 (phospho Y315) antibody - other tools:
|
Contact Abcam with a Technical Enquiry about ZAP70 (phospho Y315) antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
