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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-ZAP70 (phospho Y315) antibody
See all ZAP70 products (28) ...
Description
Rabbit polyclonal to ZAP70 (phospho Y315)
Specificity
Detects endogenous levels of ZAP70 only when phosphorylated at tyrosine 315.
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of tyrosine 315 (S-V-YP-E-S).
Positive control
Lymph node tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Adaptive Immunity >> T Cells >> CD
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ZAP70 (phospho Y315) antibody (ab60970)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab60970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
Target
Function
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Tissue specificity
Expressed in T- and natural killer cells.
Involvement in disease
Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.
Domain
The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
Post-translational
modifications
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Cellular localization
Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
Target information above from: UniProt accessionP43403
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 2.7.10.2 antibody
see all
Database links
- Entrez Gene: 7535 Human
- Entrez Gene: 22637 Mouse
- Omim: 176947 Human
- SwissProt: P43403 Human
- SwissProt: P43404 Mouse
- Unigene: 234569 Human
- Unigene: 8038 Mouse
Anti-ZAP70 (phospho Y315) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ZAP70 (phospho Y315) antibody (ab60970)
ab60970 at 1/50 - 1/100 dilution staining ZAP70 in human lymph node by immunohistochemistry on paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
References for Anti-ZAP70 (phospho Y315) antibody (ab60970)
ab60970 has not yet been referenced specifically in any publications.
Publishing research using ab60970? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"