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Western blot - ZBTB20 antibody (ab48889)
Western blot - ZBTB20 antibody (ab48889)
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Product Name 

ZBTB20 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ZBTB20

Immunogen 

A region within synthetic peptide: ERSNEVEMDS TVITVSNSSD KSVLQQPSVN TSIGQPLPST QLYLRQTETL, corresponding to internal sequence amino acids 361-410 of Human ZBTB20
BLAST 'ERSNEVEMDS TVITVSNSSD KSVLQQPSVN TSIGQPLPST QLYLRQTETL ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
(ELISA titre using peptide based assay: 1:312500)
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat cell lysate

Cellular localization 

Nuclear

Research areas 

Cancer >> Tumor biomarkers >> Oncoproteins
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

ZBTB20 is a 733-residue protein with a BTB/POZ domain at the N-terminal and 4 C2H2 zinc fingers at C-terminal. It may be a transcription factor involved in hematopoiesis, oncogenesis, and immune responses. It is expressed in spleen, lymph node, thymus, peripheral blood leukocytes, and fetal liver.

 

Alternative names ZBTB20 antibody (ab48889)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

26137    (Human)

GeneCard

    

GC03M115540    (Human)

SwissProt

    

Q9HC78    (Human)
Q8N6R5    (Human)

Unigene

    

122417    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZBTB20 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ZBTB20 antibody images, references, products related to ab48889 and other tools.

 

ZBTB20 antibody images:

Product image  Western blot - ZBTB20 antibody (ab48889)

Western blot - ZBTB20 antibody (ab48889)

ZBTB20 antibody (ab48889) at 2 µg/ml + Jurkat cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
developed using the ECL technique

Predicted band size : 73 kDa
Observed band size : 73 kDa


Diluted in 5% skim milk / PBS buffer, gel concentration 12%

 
 

Search PubMed (MEDLINE) for references to ZBTB20

 

ZBTB20 antibody - more information

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ZBTB20 antibody - related products:

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Loading controls

Lysates

 

Positive Controls for ZBTB20 antibody

ab14844

  

Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate

 

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