|
|
|
|
Western blot - ZBTB20 antibody (ab48889)

(enlarge)
|
|
|
|
|
Product Name
|
ZBTB20 antibody
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to ZBTB20
|
|
Immunogen
|
A region within synthetic peptide: ERSNEVEMDS TVITVSNSSD KSVLQQPSVN TSIGQPLPST QLYLRQTETL, corresponding to internal sequence amino acids 361-410 of Human ZBTB20 BLAST 'ERSNEVEMDS TVITVSNSSD KSVLQQPSVN TSIGQPLPST QLYLRQTETL ' with or 
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions ELISA: Use at an assay dependent dilution. (ELISA titre using peptide based assay: 1:312500) WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Jurkat cell lysate
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Cancer >> Tumor biomarkers >> Oncoproteins Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
|
|
Relevance
|
ZBTB20 is a 733-residue protein with a BTB/POZ domain at the N-terminal and 4 C2H2 zinc fingers at C-terminal. It may be a transcription factor involved in hematopoiesis, oncogenesis, and immune responses. It is expressed in spleen, lymph node, thymus, peripheral blood leukocytes, and fetal liver.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Protein A purified
|
|
Storage buffer
|
Preservative: None Constituents: 2% Sucrose, PBS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZBTB20 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for ZBTB20 antibody images, references, products related to ab48889 and other tools.
|
| |
ZBTB20 antibody images:
|
Western blot - ZBTB20 antibody (ab48889)
ZBTB20 antibody (ab48889) at 2 µg/ml + Jurkat cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution developed using the ECL technique
Predicted band size : 73 kDa Observed band size : 73 kDa
Diluted in 5% skim milk / PBS buffer, gel concentration 12%
|
| |
| |
|
Search PubMed (MEDLINE) for references to ZBTB20
|
| |
ZBTB20 antibody - more information
|
Customer reviews (feedback) regarding ZBTB20 antibody |
Customer FAQs regarding ZBTB20 antibody |
Protocols for ZBTB20 antibody |
Price and availability of products related to ZBTB20 antibody |
| |
ZBTB20 antibody - related products:
|
|
|
| |
ZBTB20 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about ZBTB20 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"