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Anti-ZBTB3 antibody (ab83481)

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Overview

Product name

Anti-ZBTB3 antibody
See all ZBTB3 products (3) ...

Description

Rabbit polyclonal to ZBTB3

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide corresponding to a region within internal sequence amino acids 525-574 (EDLAKRSKPD PEVGPLLGVQ PLPGSPTADR QSSSGGGPPK DFVLAPKTNI) of Human ZBTB3 (NP_079060).

EDLAKRSKPD PEVGPLLGVQ PLPGSPTADR QSSSGGGPPK DFVLAPKTNI

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Hela cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

  • Western blot - ZBTB3 antibody (ab83481)Western blot - ZBTB3 antibody (ab83481) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab83481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

May be involved in transcriptional regulation.

Sequence similarities

Contains 1 BTB (POZ) domain.
Contains 2 C2H2-type zinc fingers.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9H5J0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Zbtb3 antibody
  • ZBTB3_HUMAN antibody
  • Zinc finger and BTB domain containing protein 3 antibody
  • Zinc finger and BTB domain-containing protein 3 antibody

Database links

Anti-ZBTB3 antibody images:

  Western blot - ZBTB3 antibody (ab83481)

Western blot - ZBTB3 antibody (ab83481)

Anti-ZBTB3 antibody (ab83481) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)


Gel concentration: 12%

References for Anti-ZBTB3 antibody (ab83481)

ab83481 has not yet been referenced specifically in any publications.

Publishing research using ab83481? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"