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Western blot - ZBTB3 antibody (ab83481)
Western blot - ZBTB3 antibody (ab83481)
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Product Name 

ZBTB3 antibody

 

See all ZBTB3 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ZBTB3

Immunogen 

Synthetic peptide corresponding to a region within internal sequence amino acids 525-574 (EDLAKRSKPD PEVGPLLGVQ PLPGSPTADR QSSSGGGPPK DFVLAPKTNI) of Human ZBTB3 (NP_079060).
BLAST 'EDLAKRSKPD PEVGPLLGVQ PLPGSPTADR QSSSGGGPPK DFVLAPKTNI ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Hela cell lysate.

Cellular localization 

Nucleus

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

ZBTB3 contains one BTB (POZ) domain and two C2H2-type zinc fingers. It may be involved in transcriptional regulation.

 

ZBTB3 antibody (ab83481)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

79842    (Human)

SwissProt

    

Q9H5J0    (Human)

Unigene

    

147554    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZBTB3 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ZBTB3 antibody images, references, products related to ab83481 and other tools.

 

ZBTB3 antibody images:

Product image  Western blot - ZBTB3 antibody (ab83481)

Western blot - ZBTB3 antibody (ab83481)

ZBTB3 antibody (ab83481) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)


Gel concentration: 12%

 
 

Search PubMed (MEDLINE) for references to ZBTB3

 

ZBTB3 antibody - more information

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ZBTB3 antibody - related products:

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Loading controls

Lysates

 

Positive Controls for ZBTB3 antibody

ab14655

  

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

 

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ZBTB3 antibody - other tools:

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