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Western blot - ZBTB3 antibody (ab83481)

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Product Name
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ZBTB3 antibody
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See all ZBTB3 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ZBTB3
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Immunogen
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Synthetic peptide corresponding to a region within internal sequence amino acids 525-574 (EDLAKRSKPD PEVGPLLGVQ PLPGSPTADR QSSSGGGPPK DFVLAPKTNI) of Human ZBTB3 (NP_079060). BLAST 'EDLAKRSKPD PEVGPLLGVQ PLPGSPTADR QSSSGGGPPK DFVLAPKTNI ' with or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/62500. WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Hela cell lysate.
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Cellular localization
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Nucleus
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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ZBTB3 contains one BTB (POZ) domain and two C2H2-type zinc fingers. It may be involved in transcriptional regulation.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZBTB3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ZBTB3 antibody images, references, products related to ab83481 and other tools.
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ZBTB3 antibody images:
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Western blot - ZBTB3 antibody (ab83481)
ZBTB3 antibody (ab83481) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 62 kDa Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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Search PubMed (MEDLINE) for references to ZBTB3
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ZBTB3 antibody - more information
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Customer reviews (feedback) regarding ZBTB3 antibody |
Customer FAQs regarding ZBTB3 antibody |
Protocols for ZBTB3 antibody |
Price and availability of products related to ZBTB3 antibody |
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ZBTB3 antibody - related products:
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ZBTB3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ZBTB3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"