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Anti-ZC3H11A antibody (ab70101) at 1/500 dilution + rat brain tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 90 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
All lanes : Anti-ZC3H11A antibody (ab70101) at 1/500 dilution
Lane 1 : ZC3H11A transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 90 kDa
Observed band size : 140 kDa (why is the actual band size different from the predicted?)
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