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Western blot - ZC3H13 antibody (ab70802)
Western blot - ZC3H13 antibody (ab70802)
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Immunoprecipitation - ZC3H13 antibody (ab70802)
Immunoprecipitation - ZC3H13 antibody (ab70802)
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Immunocytochemistry/ Immunofluorescence - ZC3H13 antibody (ab70802)
Immunocytochemistry/ Immunofluorescence - ZC3H13 antibody (ab70802)
(enlarge)


Product Name 

ZC3H13 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ZC3H13

Immunogen 

Synthetic peptide corresponding to a region between residues 1175 and 1225 of Human ZC3H13 (NP_055885.2)

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ICC/IF, IP, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• ICC/IF

1 Abreview

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ICC/IF
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/400.
IP: Use at 2µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 197 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole cell lysate from HeLa, 293T and NIH3T3 cells.

Research areas 

Cell Biology >> Other Antibodies >> Other Antibodies

Relevance 

ZC3H13, also known as Zinc finger CCCH-type containing 13, is a novel gene whose function is still not completely clarified.

 

ZC3H13 antibody (ab70802)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

23091    (Human)
67302    (Mouse)

SwissProt

    

Q5T200    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZC3H13 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ZC3H13 antibody images, references, products related to ab70802 and other tools.

 

ZC3H13 antibody images:

Product image  Western blot - ZC3H13 antibody (ab70802)

Western blot - ZC3H13 antibody (ab70802)

All lanes : ZC3H13 antibody (ab70802) at 0.04 µg/ml

Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg

developed using the ECL technique

Predicted band size : 197 kDa
Observed band size : 240 kDa (why is the actual band size different from the predicted?)
Additional bands at : 200 kDa,85 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 3 minutes

 

Product image  Immunoprecipitation - ZC3H13 antibody (ab70802)

Immunoprecipitation - ZC3H13 antibody (ab70802)

Detection of ZC3H13 by Western Blot of Immunoprecipitate.
ab70802 at 1µg/ml staining ZC3H13 in HeLa whole cell lysate previously immunoprecipitated using ab70802 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with exposure time of 30 seconds.

 

Product image  Immunocytochemistry/ Immunofluorescence - ZC3H13 antibody (ab70802)

Immunocytochemistry/ Immunofluorescence - ZC3H13 antibody (ab70802)

ab70802 (1/400) staining ZC3H13 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% TritonX100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

This image is part of an abreview submitted by Dr. Kirk McManus, University of Manitoba/MICB, Canada

See Abreview

 
 

Search PubMed (MEDLINE) for references to ZC3H13

 

ZC3H13 antibody - more information

Customer reviews (feedback) regarding ZC3H13 antibody

Customer FAQs regarding ZC3H13 antibody

Protocols for ZC3H13 antibody

Price and availability of products related to ZC3H13 antibody

 

ZC3H13 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for ZC3H13 antibody

ab7179

  

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

ab29545

  

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

 

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ab6564

  

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ab6717

  

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ab6720

  

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ab7007

  

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ab7019

  

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10nm Gold antibody

ab27235

  

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ab27238

  

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ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

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ZC3H13 antibody - other tools:

Contact Abcam with a Technical Enquiry about ZC3H13 antibody

 

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