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Anti-ZC3H13 antibody (ab70802)

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Overview

Product name

Anti-ZC3H13 antibody
See all ZC3H13 products (2) ...

Description

Rabbit polyclonal to ZC3H13

Tested applications

ICC/IF, IP, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic peptide corresponding to a region between residues 1175 and 1225 of Human ZC3H13 (NP_055885.2)

Positive control

Whole cell lysate from HeLa, 293T and NIH3T3 cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - ZC3H13 antibody (ab70802)Western blot - ZC3H13 antibody (ab70802) image (enlarge)

  • Immunoprecipitation - ZC3H13 antibody (ab70802)Immunoprecipitation - ZC3H13 antibody (ab70802) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - ZC3H13 antibody (ab70802)Immunocytochemistry/ Immunofluorescence - ZC3H13 antibody (ab70802) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab70802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

ZC3H13, also known as Zinc finger CCCH-type containing 13, is a novel gene whose function is still not completely clarified.

Alternative names

  • 3110050K21Rik antibody
  • 4930570G11Rik antibody
  • C87618 antibody
  • KIAA0853 antibody
  • 2600010B19Rik antibody
  • DKFZp434D1812 antibody
  • FLJ35669 antibody
  • zinc finger CCCH type containing 13 antibody
see all

Anti-ZC3H13 antibody images:

  Western blot - ZC3H13 antibody (ab70802)

Western blot - ZC3H13 antibody (ab70802)

All lanes : Anti-ZC3H13 antibody (ab70802) at 0.04 µg/ml

Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg

developed using the ECL technique

Predicted band size : 197 kDa
Observed band size : 240 kDa (why is the actual band size different from the predicted?)
Additional bands at : 200 kDa,85 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 3 minutes

  Immunoprecipitation - ZC3H13 antibody (ab70802)

Immunoprecipitation - ZC3H13 antibody (ab70802)

Detection of ZC3H13 by Western Blot of Immunoprecipitate.
ab70802 at 1µg/ml staining ZC3H13 in HeLa whole cell lysate previously immunoprecipitated using ab70802 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with exposure time of 30 seconds.

  Immunocytochemistry/ Immunofluorescence - ZC3H13 antibody (ab70802)

Immunocytochemistry/ Immunofluorescence - ZC3H13 antibody (ab70802)

ab70802 (1/400) staining ZC3H13 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% TritonX100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

This image is part of an abreview submitted by Dr. Kirk McManus, University of Manitoba/MICB, Canada

See Abreview

References for Anti-ZC3H13 antibody (ab70802)

ab70802 has not yet been referenced specifically in any publications.

Publishing research using ab70802? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"