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Western blot - ZC3H13 antibody (ab70802)
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Immunoprecipitation - ZC3H13 antibody (ab70802)
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Immunocytochemistry/ Immunofluorescence - ZC3H13 antibody (ab70802)
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Product Name
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ZC3H13 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ZC3H13
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Immunogen
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Synthetic peptide corresponding to a region between residues 1175 and 1225 of Human ZC3H13 (NP_055885.2)
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/400.
IP: Use at 2µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 197 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole cell lysate from HeLa, 293T and NIH3T3 cells.
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Research areas
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Cell Biology >> Other Antibodies >> Other Antibodies
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Relevance
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ZC3H13, also known as Zinc finger CCCH-type containing 13, is a novel gene whose function is still not completely clarified.
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ZC3H13 antibody (ab70802) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZC3H13 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ZC3H13 antibody images, references, products related to ab70802 and other tools.
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ZC3H13 antibody images:
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 Western blot - ZC3H13 antibody (ab70802)
All lanes : ZC3H13 antibody (ab70802) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 197 kDa
Observed band size : 240 kDa (why is the actual band size different from the predicted?)
Additional bands at : 200 kDa,85 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
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Immunoprecipitation - ZC3H13 antibody (ab70802)
Detection of ZC3H13 by Western Blot of Immunoprecipitate.
ab70802 at 1µg/ml staining ZC3H13 in HeLa whole cell lysate previously immunoprecipitated using ab70802 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with exposure time of 30 seconds.
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Immunocytochemistry/ Immunofluorescence - ZC3H13 antibody (ab70802)
ab70802 (1/400) staining ZC3H13 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% TritonX100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details. This image is part of an abreview submitted by Dr. Kirk McManus, University of Manitoba/MICB, Canada
See Abreview
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Search PubMed (MEDLINE) for references to ZC3H13
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ZC3H13 antibody - more information
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Customer reviews (feedback) regarding ZC3H13 antibody |
Customer FAQs regarding ZC3H13 antibody |
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Price and availability of products related to ZC3H13 antibody |
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ZC3H13 antibody - related products:
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ZC3H13 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ZC3H13 antibody |
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