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Anti-ZC3H14 antibody (ab69047)

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Overview

Product name

Anti-ZC3H14 antibody
See all ZC3H14 products (3) ...

Description

Mouse polyclonal to ZC3H14

Tested applications

ICC/IF, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length ZC3H14 protein (Human) (NP_997545)

Positive control

ZC3H14 transfected 293T cell lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 1X PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - ZC3H14 antibody (ab69047)Western blot - ZC3H14 antibody (ab69047) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab69047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent concentration. (PubMed: 21734151)

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).

Target

Relevance

ZC3H14 belongs to the ZC3H14 family. It contains two C3H1-type zinc fingers. It binds the polyadenosine RNA oligonucleotides.

Cellular localization

Nuclear

Alternative names

  • FLJ11806 antibody
  • MGC26892 antibody
  • Nuclear protein UKp68 antibody
  • NYREN 37 antibody
  • Renal carcinoma antigen NYREN 37 antibody
  • UKp68 antibody
  • Zinc finger CCCH type containing 14 antibody
see all

Database links

Anti-ZC3H14 antibody images:

  Western blot - ZC3H14 antibody (ab69047)

Western blot - ZC3H14 antibody (ab69047)

All lanes : Anti-ZC3H14 antibody (ab69047) at 1/500 dilution

Lane 1 : ZC3H14 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate

Lysates/proteins at 25 µg per lane.

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size : 35 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)

References for Anti-ZC3H14 antibody (ab69047)

This product has been referenced in:

  • Pak Cet al. Mutation of the conserved polyadenosine RNA binding protein, ZC3H14/dNab2, impairs neural function in Drosophila and humans. Proc Natl Acad Sci U S A : (2011). WB, ICC/IF; Human.Read more (PubMed: 21734151) »

See 1 publication for this product

Publishing research using ab69047? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"