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Overview
Product name
Anti-ZC3H14 antibody
See all ZC3H14 products (3) ...
Description
Mouse polyclonal to ZC3H14
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length ZC3H14 protein (Human) (NP_997545)
Positive control
ZC3H14 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
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Western blot - ZC3H14 antibody (ab69047)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 21734151)
WB: 1/500 - 1/1000.Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).
Target
Relevance
ZC3H14 belongs to the ZC3H14 family. It contains two C3H1-type zinc fingers. It binds the polyadenosine RNA oligonucleotides.
Cellular localization
Nuclear
Alternative names
- FLJ11806 antibody
- MGC26892 antibody
- Nuclear protein UKp68 antibody
see all
Database links
- Entrez Gene: 79882 Human
- SwissProt: Q6PJT7 Human
Anti-ZC3H14 antibody images:
Western blot - ZC3H14 antibody (ab69047)
All lanes : Anti-ZC3H14 antibody (ab69047) at 1/500 dilution
Lane 1 : ZC3H14 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 35 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
References for Anti-ZC3H14 antibody (ab69047)
This product has been referenced in:
- Pak Cet al. Mutation of the conserved polyadenosine RNA binding protein, ZC3H14/dNab2, impairs neural function in Drosophila and humans. Proc Natl Acad Sci U S A : (2011). WB, ICC/IF; Human.Read more (PubMed: 21734151) »
See 1 publication for this product
Publishing research using ab69047? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"