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Overview
Product name
Anti-ZCCHC10 antibody
See all ZCCHC10 products (2) ...
Description
Mouse polyclonal to ZCCHC10
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length (aa 1-170) human ZCCHC10 isoform 2 (NP_060135). ,
Positive control
ZCCHC10 transfected 293T lysate; HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
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Western blot - ZCCHC10 antibody (ab71844)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ICC/IF
Application notes
ICC/IF: Use at a concentration of 10 µg/ml.
Note: ab71844 requires purification for working in this application.
WB: 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 18 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Sequence similarities
Contains 1 CCHC-type zinc finger.
Target information above from: UniProt accessionQ8TBK6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
There are 2 isoforms produced by alternative splicing.
Alternative names
- 2410141K03Rik antibody
- AA675321 antibody
- D11Ertd416e antibody
see all
Database links
- Entrez Gene: 54819 Human
- SwissProt: Q8TBK6 Human
- Unigene: 29700 Human
Anti-ZCCHC10 antibody images:
Western blot - ZCCHC10 antibody (ab71844)
All lanes : Anti-ZCCHC10 antibody (ab71844) at 1/500 dilution
Lane 1 : ZCCHC10 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 18 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
References for Anti-ZCCHC10 antibody (ab71844)
ab71844 has not yet been referenced specifically in any publications.
Publishing research using ab71844? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"