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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Anti-ZCCHC12 antibody
See all ZCCHC12 products (3) ...
Rabbit polyclonal to ZCCHC12
Reacts with
Rat, Human
Predicted to work with
Mouse, Cow, Dog
A region within synthetic peptide: AREVMRVLQA TNPNLSVADF LRAMKLVFGE SESSVTAHGK FFNTLQAQGE, corresponding to amino acids 94-143 of Human ZCCHC12
AREVMRVLQA TNPNLSVADF LRAMKLVFGE SESSVTAHGK FFNTLQAQGE
HepG2 cell lysate
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
ab74774 is purified by peptide affinity chromatography method.
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> SMADs
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> SMADs
Signal Transduction >> Growth Factors/Hormones >> TGF
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Western blot - ZCCHC12 antibody (ab74774)
(enlarge)
Our Abpromise guarantee covers the use of ab74774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.
ELISA titre using peptide based assay: 1:1562500
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ZCCHC12 contains 1 CCHC type zinc finger. ZCCHC12 is the transcriptional coactivator in the bone morphogenetic protein (BMP) signaling pathway. It positively modulates BMP signaling by interacting with SMAD1 and associating with CBP in the transcription complex. It contributes to the BMP induced enhancement of cholinergic neuron specific gene expression.
Western blot - ZCCHC12 antibody (ab74774)

Anti-ZCCHC12 antibody (ab74774) at 1 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 45 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
ab74774 has not yet been referenced specifically in any publications.
Publishing research using ab74774? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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Anti-ZCCHC12 antibody (ab74774) at 1 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 45 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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