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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-ZCCHC12 antibody
See all ZCCHC12 products (3) ...
Description
Rabbit polyclonal to ZCCHC12
Tested applications
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Cow, Dog
Immunogen
A region within synthetic peptide: AREVMRVLQA TNPNLSVADF LRAMKLVFGE SESSVTAHGK FFNTLQAQGE, corresponding to amino acids 94-143 of Human ZCCHC12
AREVMRVLQA TNPNLSVADF LRAMKLVFGE SESSVTAHGK FFNTLQAQGE
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab74774 is purified by peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> SMADs
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> SMADs
Signal Transduction >> Growth Factors/Hormones >> TGF
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Western blot - ZCCHC12 antibody (ab74774)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.
ELISA titre using peptide based assay: 1:1562500
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ZCCHC12 contains 1 CCHC type zinc finger. ZCCHC12 is the transcriptional coactivator in the bone morphogenetic protein (BMP) signaling pathway. It positively modulates BMP signaling by interacting with SMAD1 and associating with CBP in the transcription complex. It contributes to the BMP induced enhancement of cholinergic neuron specific gene expression.
Alternative names
- FLJ16123 antibody
- SIZN antibody
- SIZN1 antibody
see all
Database links
- Entrez Gene: 540006 Cow
- Entrez Gene: 170261 Human
- Entrez Gene: 72693 Mouse
- Entrez Gene: 313436 Rat
- Omim: 300701 Human
- SwissProt: Q08DL1 Cow
- SwissProt: Q6PEW1 Human
- SwissProt: Q6PID5 Human
see all
Anti-ZCCHC12 antibody images:
Western blot - ZCCHC12 antibody (ab74774)
Anti-ZCCHC12 antibody (ab74774) at 1 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 45 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
References for Anti-ZCCHC12 antibody (ab74774)
ab74774 has not yet been referenced specifically in any publications.
Publishing research using ab74774? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"