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Western blot - ZCCHC12 antibody (ab74774)
(enlarge)
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Product Name
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ZCCHC12 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ZCCHC12
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Immunogen
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A synthetic peptide corresponding to a region of human ZCCHC12
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Reacts with
(species key)
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Hu, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.
ELISA titre using peptide based assay: 1:1562500
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> SMADs
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> SMADs
Signal Transduction >> Growth Factors/Hormones >> TGF
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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ZCCHC12 contains 1 CCHC type zinc finger. ZCCHC12 is the transcriptional coactivator in the bone morphogenetic protein (BMP) signaling pathway. It positively modulates BMP signaling by interacting with SMAD1 and associating with CBP in the transcription complex. It contributes to the BMP induced enhancement of cholinergic neuron specific gene expression.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Purification notes
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ab74774 is purified by peptide affinity chromatography method.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZCCHC12 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ZCCHC12 antibody images, references, products related to ab74774 and other tools.
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ZCCHC12 antibody images:
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 Western blot - ZCCHC12 antibody (ab74774)
ZCCHC12 antibody (ab74774) at 1 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 45 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to ZCCHC12
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ZCCHC12 antibody - more information
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Customer reviews (feedback) regarding ZCCHC12 antibody |
Customer FAQs regarding ZCCHC12 antibody |
Protocols for ZCCHC12 antibody |
Price and availability of products related to ZCCHC12 antibody |
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ZCCHC12 antibody - related products:
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ZCCHC12 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ZCCHC12 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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