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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab76901? Please let us know so that we can cite the reference in this datasheet
ab76901 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - ZCCHC6 antibody (ab76901)
Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml + human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 171 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Western blot - ZCCHC6 antibody (ab76901)
All lanes : Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml
Lane 1 : ZCCHC6 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 171 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)
Note: predicted molecular weight for isoform 5 is 48 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ZCCHC6 antibody (ab76901)
ab76901 staining ZCCHC6 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in 0.1 sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining method was used for detection.
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