Anti-ZCCHC6 antibody (ab76901)
Key features and details
- Mouse polyclonal to ZCCHC6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZCCHC6 antibody
See all ZCCHC6 primary antibodies -
Description
Mouse polyclonal to ZCCHC6 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length human ZCCHC6 protein (aa 1-412 of AAH32456 or Swissprot id Q5VYS8-5).
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Positive control
- Human liver tissue lysate; ZCCHC6 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 171, 48 kDa.
Predicted molecular weight, isoform 1: 171 kDa, isoform 5: 48 kDa. |
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IHC-P |
1/50.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 171, 48 kDa. Predicted molecular weight, isoform 1: 171 kDa, isoform 5: 48 kDa. |
IHC-P
1/50. |
Target
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Relevance
ZCCHC6 contains 3 CCHC-type zinc fingers. -
Database links
- Entrez Gene: 79670 Human
- Omim: 613467 Human
- SwissProt: Q5VYS8 Human
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Alternative names
- KIAA1711 antibody
- MGC38585 antibody
- PAP associated domain containing 6 antibody
see all
Images
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Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml + human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 171, 48 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
All lanes : Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml
Lane 1 : ZCCHC6 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 171, 48 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Note: predicted molecular weight for isoform 5 is 48 kDa. -
ab76901 staining ZCCHC6 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in 0.1 sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining method was used for detection.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab76901 has not yet been referenced specifically in any publications.