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Overview
Product name
Anti-ZCCHC6 antibody
Description
Mouse polyclonal to ZCCHC6
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length human ZCCHC6 protein (aa 1-412 of AAH32456 or Swissprot id Q5VYS8-5).
Positive control
Human liver tissue lysate; ZCCHC6 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: 1/50.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight, isoform 1: 171 kDa, isoform 5: 48 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ZCCHC6 contains 3 CCHC-type zinc fingers.
Alternative names
- KIAA1711 antibody
- MGC38585 antibody
- PAPD6 antibody
see all
Database links
- Entrez Gene: 79670 Human
- SwissProt: Q5VYS8 Human
Anti-ZCCHC6 antibody images:
Western blot - ZCCHC6 antibody (ab76901)
Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml + human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 171 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Western blot - ZCCHC6 antibody (ab76901)
All lanes : Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml
Lane 1 : ZCCHC6 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 171 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)
Note: predicted molecular weight for isoform 5 is 48 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ZCCHC6 antibody (ab76901)
ab76901 staining ZCCHC6 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in 0.1 sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining method was used for detection.
References for Anti-ZCCHC6 antibody (ab76901)
ab76901 has not yet been referenced specifically in any publications.
Publishing research using ab76901? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"