Anti-ZCH11 antibody (ab89165)
Key features and details
- Mouse polyclonal to ZCH11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZCH11 antibody
See all ZCH11 primary antibodies -
Description
Mouse polyclonal to ZCH11 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human ZCH11. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human spleen tissue lysate; ZCH11 transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 185 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 185 kDa. |
Target
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Function
Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. -
Sequence similarities
Belongs to the DNA polymerase type-B-like family.
Contains 3 CCHC-type zinc fingers.
Contains 2 PAP-associated domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS. - Information by UniProt
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Database links
- Entrez Gene: 23318 Human
- Omim: 613692 Human
- SwissProt: Q5TAX3 Human
- Unigene: 655407 Human
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Alternative names
- DKFZp779C1943 antibody
- EC 2.7.7.52 antibody
- FLJ42878 antibody
see all
Images
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Anti-ZCH11 antibody (ab89165) at 1 µg/ml + Human spleen tissue lysate at 50 µg
Predicted band size: 185 kDa
Observed band size: 185 kDa -
All lanes : Anti-ZCH11 antibody (ab89165) at 1 µg/ml
Lane 1 : ZCH11 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 185 kDa
Datasheets and documents
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Datasheet download
References (1)
ab89165 has been referenced in 1 publication.
- Plé H et al. The repertoire and features of human platelet microRNAs. PLoS One 7:e50746 (2012). WB ; Human . PubMed: 23226537