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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-butyrate response factor 1 antibody
See all butyrate response factor 1 products (7) ...
Description
Rabbit polyclonal to butyrate response factor 1
Tested applications
ELISA, WB, EMSAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
Synthetic peptide from Human butyrate response factor 1
Positive control
Human placenta lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - ZFP36L1 antibody (ab42473)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/62,500ELISA: 1/62,500
WB: Use a concentration of 0.125 µg/mlDetects a band of approximately 30 kDa (predicted molecular weight: 36 kDa).
EMSA: Use at an assay dependent dilution. (PubMed: 20691152)
Target
Relevance
Butyrate response factor 1 is a member of the TIS11 family of early response genes. Family members are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. The gene is well conserved across species and has a promoter that contains motifs seen in other early response genes. The encoded protein contains a distinguishing putative zinc finger domain with a repeating Cys-His motif. This putative nuclear transcription factor probably functions in regulating the response to growth factors.
Cellular localization
Nuclear
Alternative names
- Berg36 antibody
- BRF1 antibody
- butyrate response factor 1 antibody
see all
Database links
- Entrez Gene: 677 Human
- Entrez Gene: 12192 Mouse
- Entrez Gene: 29344 Rat
- Omim: 601064 Human
- SwissProt: Q07352 Human
- SwissProt: P23950 Mouse
- SwissProt: P17431 Rat
- Unigene: 85155 Human
see all
Anti-butyrate response factor 1 antibody images:
Western blot - ZFP36L1 antibody (ab42473)
Anti-butyrate response factor 1 antibody (ab42473) at 0.125 µg/ml (5% skimmed milk / PBS) + 10µg Human placenta whole cell lysate
Secondary
HRP conjugated anti-rabbit at 1/50000 dilution
Predicted band size : 36 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%.
References for Anti-butyrate response factor 1 antibody (ab42473)
This product has been referenced in:
- Kim CWet al. Ectopic over-expression of tristetraprolin in human cancer cells promotes biogenesis of let-7 by down-regulation of Lin28. Nucleic Acids Res : (2011). WB; Human.Read more (PubMed: 22210895) »
- Kim CWet al. Tristetraprolin controls the stability of cIAP2 mRNA through binding to the 3'UTR of cIAP2 mRNA. Biochem Biophys Res Commun 400:46-52 (2010). EMSA; Human.Read more (PubMed: 20691152) »
See all 3 publications for this product
Publishing research using ab42473? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"