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Anti-ZIP Kinase antibody [EPR1635] (ab79422)

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Overview

Product name

Anti-ZIP Kinase antibody [EPR1635]
See all ZIP Kinase products (7) ...

Description

Rabbit monoclonal [EPR1635] to ZIP Kinase

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues on the N terminal of Human ZIP Kinase.

Positive control

HeLa and A431 cell lysates.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EPR1635

Isotype

IgG

Research areas

Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases

  • Western blot - ZIP Kinase antibody [EPR1635] (ab79422)Western blot - ZIP Kinase antibody [EPR1635] (ab79422) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab79422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/5000 - 1/10000.Det...Read more →

    WB: 1/5000 - 1/10000.Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Application notes

Is unsuitable for ,Flow Cyt,ICC,IHC-P or IP.

Target

Function

Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.

Post-translational
modifications

Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
Autophosphorylated. Phosphorylated by ROCK1.

Cellular localization

Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.

Target information above from: UniProt accessionO43293 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DAP kinase 3 antibody
  • DAP like kinase antibody
  • DAP-like kinase antibody
  • Dapk 3 antibody
  • Dapk3 antibody
  • DAPK3_HUMAN antibody
  • Death associated kinase 3 antibody
  • Death associated protein kinase 3 antibody
  • Death-associated protein kinase 3 antibody
  • Dlk antibody
  • EC 2.7.11.1 antibody
  • FLJ36473 antibody
  • ZIP antibody
  • ZIP kinase antibody
  • ZIP-kinase antibody
  • ZIPK antibody
see all

Database links

Anti-ZIP Kinase antibody [EPR1635] images:

  Western blot - ZIP Kinase antibody [EPR1635] (ab79422)

Western blot - ZIP Kinase antibody [EPR1635] (ab79422)

All lanes : Anti-ZIP Kinase antibody [EPR1635] (ab79422) at 1/5000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 53 kDa
Observed band size : 53 kDa

References for Anti-ZIP Kinase antibody [EPR1635] (ab79422)

ab79422 has not yet been referenced specifically in any publications.

Publishing research using ab79422? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"