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Western blot - ZIP Kinase antibody (ab2057)
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Immunocytochemistry/ Immunofluorescence - ZIP Kinase antibody (ab2057)
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Product Name
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ZIP Kinase antibody
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See all ZIP Kinase antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ZIP Kinase
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Immunogen
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Synthetic peptide.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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It has no cross reactivity to DAP kinase.
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Epitope
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RRRNVRGEDSGRKPERRRLK
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
Western blot at 1:1000 dilution and a 52kDa band should be detected.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate or Jurkat whole cell lysate.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Relevance
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Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certainserine/threonine protein kinases, such as ASK1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N terminal kinase domain and a C terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREBfamily. ZIP kinase has high sequence homology to DAP kinase (death associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.
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ZIP Kinase antibody (ab2057) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Apoptosis inducing kinase.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ZIP Kinase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ZIP Kinase antibody images, references, products related to ab2057 and other tools.
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ZIP Kinase antibody images:
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 Immunocytochemistry/ Immunofluorescence - ZIP Kinase antibody (ab2057)
ab2057 at 10µg/ml staining of ZIP kinase in Jurkat cell by ICC/IF
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References for ZIP Kinase antibody (ab2057)
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This product has been used in:
Puto LA & Reed JC Daxx represses RelB target promoters via DNA methyltransferase recruitment and DNA hypermethylation. Genes Dev 22:998-1010 (2008). PubMed: 18413714
If you publish research using ab2057 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ZIP Kinase
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ZIP Kinase antibody - more information
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Customer reviews (feedback) regarding ZIP Kinase antibody |
Customer FAQs regarding ZIP Kinase antibody |
Protocols for ZIP Kinase antibody |
Price and availability of products related to ZIP Kinase antibody |
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ZIP Kinase antibody - related products:
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ZIP Kinase antibody - other tools:
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Contact Abcam with a Technical Enquiry about ZIP Kinase antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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