Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-ZIP Kinase antibody
See all ZIP Kinase products (7) ...
Description
Rabbit polyclonal to ZIP Kinase
Specificity
It has no cross reactivity to DAP kinase.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide.
Epitope
RRRNVRGEDSGRKPERRRLK
Positive control
HeLa whole cell lysate or Jurkat whole cell lysate.
General notes
Apoptosis inducing kinase.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 10 µg/ml.
Western blot at 1:1000 dilution and a 52kDa band should be detected.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
Autophosphorylated. Phosphorylated by ROCK1.
Cellular localization
Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
Target information above from: UniProt accessionO43293
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAP kinase 3 antibody
- DAP like kinase antibody
- DAP-like kinase antibody
see all
Database links
- Entrez Gene: 1613 Human
- Entrez Gene: 13144 Mouse
- Entrez Gene: 64391 Rat
- Omim: 603289 Human
- SwissProt: O43293 Human
- SwissProt: O54784 Mouse
- SwissProt: O88764 Rat
- Unigene: 631844 Human
see all
Anti-ZIP Kinase antibody images:
Western blot - ZIP Kinase antibody (ab2057)
All lanes : Anti-ZIP Kinase antibody (ab2057) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - ZIP Kinase antibody (ab2057)
ab2057 at 10µg/ml staining of ZIP kinase in Jurkat cell by ICC/IF
References for Anti-ZIP Kinase antibody (ab2057)
This product has been referenced in:
- Puto LA & Reed JC Daxx represses RelB target promoters via DNA methyltransferase recruitment and DNA hypermethylation. Genes Dev 22:998-1010 (2008).Read more (PubMed: 18413714) »
See 1 publication for this product
Publishing research using ab2057? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"