ZIP Kinase (phospho T265) antibody (ab63395)

Product Name 

ZIP Kinase (phospho T265) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ZIP Kinase (phospho T265)

Immunogen 

A synthesized phosphopeptide derived from human ZIP Kinase around the phosphorylation site of threonine 265 (RMT^PIA).

Reacts with 
(species key)

Hu, Ms

Specificity 

This antibody detects endogenous levels of ZIP kinase only when phosphorylated at threonine 265.

Tested applications 
(see key)

ELISA, ICC/IF, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A549 cells and extracts from HuvEc cells

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases

Relevance 

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certainserine/threonine protein kinases, such as ASK1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N terminal kinase domain and a C terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREBfamily. ZIP kinase has high sequence homology to DAP kinase (death associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg⁺² and Ca⁺²), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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