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Anti-ZIP Kinase (phospho T265) antibody (ab63395)

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Overview

Product name

Anti-ZIP Kinase (phospho T265) antibody
See all ZIP Kinase products (7) ...

Description

Rabbit polyclonal to ZIP Kinase (phospho T265)

Specificity

This antibody detects endogenous levels of ZIP kinase only when phosphorylated at threonine 265.

Tested applications

WB, ICC/IF, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

A synthesized phosphopeptide derived from human ZIP Kinase around the phosphorylation site of threonine 265 (RMTPIA).

Positive control

A549 cells and extracts from HuvEc cells

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases

  • Immunocytochemistry/ Immunofluorescence - ZIP Kinase (phospho T265) antibody (ab63395)Immunocytochemistry/ Immunofluorescence - ZIP Kinase (phospho T265) antibody (ab63395) image (enlarge)

  • Western blot - ZIP Kinase (phospho T265) antibody (ab63395)Western blot - ZIP Kinase (phospho T265) antibody (ab63395) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab63395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

  • 1 Image

    ICC/IF

     ICC/IF: 1/500 - 1/1000.

    ICC/IF: 1/500 - 1/1000.

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

Target

Function

Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.

Post-translational
modifications

Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
Autophosphorylated. Phosphorylated by ROCK1.

Cellular localization

Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.

Target information above from: UniProt accessionO43293 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DAP kinase 3 antibody
  • DAP like kinase antibody
  • DAP-like kinase antibody
  • Dapk 3 antibody
  • DAPK3 antibody
  • DAPK3_HUMAN antibody
  • Death associated kinase 3 antibody
  • Death associated protein kinase 3 antibody
  • Death-associated protein kinase 3 antibody
  • Dlk antibody
  • EC 2.7.11.1 antibody
  • FLJ36473 antibody
  • ZIP antibody
  • ZIP kinase antibody
  • ZIP-kinase antibody
  • ZIPK antibody
see all

Database links

Anti-ZIP Kinase (phospho T265) antibody images:

  Immunocytochemistry/ Immunofluorescence - ZIP Kinase (phospho T265) antibody (ab63395)

Immunocytochemistry/ Immunofluorescence - ZIP Kinase (phospho T265) antibody (ab63395)

Immunofluorescence analysis of A549 cells, using ab63395 at a 1:500.
Left image untreated.
Right image treated with phospho peptide.

  Western blot - ZIP Kinase (phospho T265) antibody (ab63395)

Western blot - ZIP Kinase (phospho T265) antibody (ab63395)

All lanes : Anti-ZIP Kinase (phospho T265) antibody (ab63395) at 1/500 dilution

Lane 1 : Extracts of HuvEc cells
Lane 2 : Extracts of HuvEc cells plus phospho petide


Predicted band size : 53 kDa
Observed band size : 53 kDa


The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.

References for Anti-ZIP Kinase (phospho T265) antibody (ab63395)

ab63395 has not yet been referenced specifically in any publications.

Publishing research using ab63395? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"