Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF161
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region from internal sequence amino acids 288-337 (

    SCNICGKLLSAAYITSHLKTHGQSQSINCNTCKQGISKTCMSEETSNQKQ

    ) of Human ZNF161 (NP_009077).

  • Positive control
    • 293T Cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Possible transcription factor. Specifically binds to the CT/GC-rich region of the interleukin-3 promoter and mediates tax transactivation of IL-3.
  • Tissue specificity
    Ubiquitously expressed. Highest levels in skeletal muscle and kidney.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DB1 antibody
    • MGC82370 antibody
    • Putative transcription factor DB1 antibody
    • Vascular endothelial zinc finger 1 antibody
    • VEZF1 antibody
    • VEZF1_HUMAN antibody
    • Zinc finger protein 161 antibody
    • ZNF161 antibody
    see all

Images

  • Anti-ZNF161 antibody (ab85414) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T Cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling ZNF161 with ab85414 at 5µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling ZNF161 with ab85414.



  • Predicted band size : 57 kDa
    Western Blot using ab85414 at 1.0ug/ml. Sample type: Hum. Adult Placenta Lysate


  • Predicted band size : 57 kDa
    Western Blot using ab85414 at 1.0ug/ml. Sample type: Hum. Fetal Liver Lysate


  • Predicted band size : 57 kDa
    Western Blot using ab85414 at 1.0ug/ml. Sample type: Hum. Fetal Lung Lysate


  • Predicted band size : 57 kDa
    Western Blot using ab85414 at 1.0ug/ml. Sample type: Hum. Fetal Muscle Lysate

References

This product has been referenced in:
  • Gerald D  et al. RhoB controls coordination of adult angiogenesis and lymphangiogenesis following injury by regulating VEZF1-mediated transcription. Nat Commun 4:2824 (2013). Read more (PubMed: 24280686) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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