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Anti-Zebrafish Gut Absorptive Cell Epitopes antibody [FIS 4E8/1] (ab73643)

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Overview

Product name

Anti-Zebrafish Gut Absorptive Cell Epitopes antibody [FIS 4E8/1]
See all Zebrafish Gut Absorptive Cell Epitopes products (2) ...

Description

Mouse monoclonal [FIS 4E8/1] to Zebrafish Gut Absorptive Cell Epitopes

Tested applications

WB, IPmore details

Cross reactivity

Reacts with

Zebrafish

Immunogen

Tissue / cell preparation (Zebrafish): Lysate of zebrafish intestines.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Primary antibody notes

The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyx at the apical surface of intestinal absorptive cells. This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.

Clonality

Monoclonal

Clone number

FIS 4E8/1

Myeloma

Sp2/0-Ag14

Isotype

IgG1

Applications

Show applications key

Our Abpromise guarantee covers the use of ab73643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     
  • IP

     

Application notes

IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This antibody is also suitable for use in histology.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

References for Anti-Zebrafish Gut Absorptive Cell Epitopes antibody [FIS 4E8/1] (ab73643)

This product has been referenced in:

  • Crosnier C  et al. Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine. Development 132:1093-104 (2005).Read more (PubMed: 15689380) »

See 1 publication for this product

Publishing research using ab73643? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"